聚甘露糖醛酸对中国对虾免疫相关酶活性和溶菌溶血活性的影响  

Effects of mannuronate polysaccharide on enzymes of Penaeus chinensis related with immune and hemolysis

作　　者：刘岩[1] 汪晓路 吕青[1] 管华诗[1] 

机构地区：[1]青岛海洋大学水产学院,山东青岛266003

出　　处：《水产学报》2000年第6期549-553,共5页Journal of Fisheries of China

基　　金：九五科技攻关项目!(96-C02-01)

摘　　要：在中国对虾的血清、肌肉和肝胰腺提取液中有酸性磷酸酶（ＡＣＰ）、碱性磷酸酶（ＡＬＰ）、过氧化物酶（ＰＯＤ），其中，ＡＣＰ和ＡＬＰ？在肝脏中的活性最高。采用１．０％的聚甘露糖醛酸多糖作为免疫药物，对中国对虾进行注射刺激后，发现血清中的溶菌和溶血活性有显著提高；肝胰腺中的ＡＣＰ和ＡＬＰ活性也有明显增加，在７２ｈ，ＡＣＰ活性由对照组的３．２１Ｕ·１００ｍＬ－１提高到９．６９Ｕ·１００ｍＬ－１，ＡＬＰ活性由４．０９Ｕ·１００ｍＬ－１提高到 １３．２Ｕ·１００ｍＬ－１，而血清和肌肉中这两种酶活性的变化不很明显。在血清、肝胰腺和肌肉中ＰＯＤ活性变化不明显。聚甘露糖醛酸多糖具有一定的增强中国对虾免疫活性的作用。Acid phosphatase (ACP), alkaline phosphatase (ALP) and peroxidase were found in the serum, meat and hepatopancreas extrace of Penaeus chinensis. The activities of ACP and ALP in the hepatopancreas were highest among the serum, meat and hepatopancreas. After Penaeus chinensis were stimulated by injection with 1. 0% polymannuronic acids, the activities of bacteriolysis and hemolysis in the serum increased, meat and the activities of ACP and ALP in the hepatopancreas enhanced obviously. ACP activity increased from 3. 21 (U. l00mL- 1 )of control group to 9.69(U. l00mL-1 ) and ALP activity increased from 4.09(U. l00mL- 1 ) to 13. 2(U' l00mL- 1 ) after 72 hours. However, the activities of these two enzymes in the serum and meat extract extract stable. The activities of POD in serum, and hepatopancreas changed little. So mannuronate polysaccharide could strengthen the immunological competence of Penaeus chinensis, and possessed some developmental value in aquatic culture.

关 键 词：中国对虾 聚甘露糖醛酸多糖 免疫活性 酶活性 

分 类 号：S942.5[农业科学—水产养殖] S945.46[农业科学—水产科学]