两种香青中总黄酮含量测定及抗氧化活性  

Determination of Total Flavones and Antioxidant Activity of Anaphalis lactea Maxim.And Anaphalis margaritacea Benth.

作　　者：王瑛[1,2] 张本印[1] 党军[1,2] 陶燕铎[1] 梅丽娟[1] 王启兰[1] 

机构地区：[1]中国科学院西北高原生物研究所,青海省西宁市新宁路23号810008 [2]中国科学院研究生院,北京市石景山区玉泉路19号100049

出　　处：《光谱实验室》2013年第5期2104-2108,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金(31101012);国家科技攻关项目(0713351614)

摘　　要：研究对乳白香青和珠光香青进行定性、定量比较分析,建立乳白香青与珠光香青总黄酮含量测定方法并进行对比分析。采用薄层层析实验对乳白香青与珠光香青中总黄酮进行定性鉴别;用紫外分光光度法,对两种香青的总黄酮含量及其抗氧化活性进行测定。经测定,乳白香青与珠光香青中总黄酮含量分别为40.43mg/mL和34.32mg/mL;乳白香青抑制DPPH自由基的能力IC50为2.96mg/mL;珠光香青I C50为3.488m g/mL。To screen the extracting method of total flavones in Anaphalis lacteal Maxin and Anaphalis margaritacea Benth,this study took different methods to make a qualitative analysis,this research provide a scientific basis for further study of optimized extracting technique of total flavones. It also make full use of Anaphalis lacteal Maxin and Anaphalis margaritacea Benth resources. This study used rutin as a comparison,applied spectrophotometry at 500 nm to determine the content of total flavones in. The qualitative analysis is mainly in chemical analysis ways. By comparing and analysis,The content of total flavones in Anaphalis lacteal Maxin （40. 43 mg/mL） relatively higher than in Anaphalis margaritacea Benth （34.32 mg/mL）.

关 键 词：珠光香青 乳白香青 总黄酮 抗氧化活性 

分 类 号：O657.32[理学—分析化学] O657.7[理学—化学]