比色法测定Annexin V变体冻干品中的氯化亚锡含量  

Colorimetric Determination of Stannous Chloride in Annexin V Derivative Kit

作　　者：蒋泉福[1] 陆春雄[1] 胡敏进[2,3] 俞惠新[1] 季宇[2,3] 华子春[2,3,4] 

机构地区：[1]江苏省原子医学研究所卫生部核医学重点实验室江苏省分子核医学重点实验室,江苏省无锡市钱荣路20号221142 [2]江苏靶标生物医药研究所有限公司 [3]常州南京大学高新技术研究院,常州市常武中路801号213164 [4]南京大学医药生物技术国家重点实验室,南京市汉口路22号210093

出　　处：《光谱实验室》2013年第5期2298-2301,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：卫生部课题(W201207);江苏省科技基础设施建设计划--科技公共服务平台(BM2012066);南京大学医药生物技术国家重点实验室开放基金资助项目(KF-GN-201304)

摘　　要：测定自制的A nnex in V变体冻干品中的氯化亚锡含量。在酸性条件下,用钼酸钠-硫氰酸钾比色法测定,K=460nm。亚锡在5—50Lg内线性关系良好,低、中、高三浓度平均回收率分别为102.9%,99.9%,101.0%;RSD分别为6.4%,8.1%,5.1%(n=6)。用比色法测定A nnex in V变体冻干品中亚锡含量,方法简便、迅捷、试剂易得,可用于冻干品中氯化亚锡的质量控制。Content of stannous chloride in annexin V derivative kit made by ourselves was determined. Sn（Ⅱ） is determinated by colorimetry with sodium molybdate and potassium thiocyanate in acid condition,The wave length was 460nm. The linearity of Sn（ Ⅱ ） was favorable in the range of 5--50μg. The Low, medium, and high average recovery was 102. 9%, 99. 9%, 101. 0% and the relative standard deviation was 6. 4%, 8. 1%, 5. 1% respectively. Colorimetric determination of stannous chloride is easily, and the result is satisfactory. It can be applied for quality control of stannous chloride in kit.

关 键 词：氯化亚锡 比色法 ANNEXIN V变体冻干品 

分 类 号：R927.2[医药卫生—药学] O657.32[理学—分析化学]