胰腺星状细胞在2型糖尿病模型大鼠胰岛纤维化的作用  

The role of pancreatic stellate cells in islet fibrosis of T2DM rat models

作　　者：李凤飞[1,2] 孙子林[1,2] 陈碧君[1,2] 殷俊梅[1,2] 翟清 查敏[1,2] 

机构地区：[1]东南大学附属中大医院内分泌科 [2]东南大学医学院糖尿病研究所,南京210009

出　　处：《中国糖尿病杂志》2013年第9期828-831,共4页Chinese Journal of Diabetes

基　　金：国家自然基金面上项目(30971399;81170716;81270010)

摘　　要：目的观察胰腺星状细胞(PSC)在T2DM模型GK大鼠胰岛中的存在状态,探讨PSC在胰岛纤维化发生发展中的作用。方法GK与Wistar大鼠胰腺冰冻切片行Masson染色和荧光双标(α-SMA/Insulin)染色。采用外向生长法分离纯化GK大鼠胰岛外生细胞,免疫荧光检测Vimentin,α-平滑肌肌动蛋白(α-SMA),Fibronectin(FN)和ColⅠ/Ⅲ的表达。结果 GK大鼠胰岛纤维化区域α-SMA染色阳性细胞增多,胰岛素分泌缺失。大量PSC自GK大鼠胰岛外生,表达Vimentin、α-SMA、FN和ColⅠ/Ⅲ。结论活化PSC存在于T2DM模型GK大鼠纤维化胰岛中,并在T2DM胰岛纤维化中发挥了一定的作用。Objective To observe pancreatic stellate cell （PSC） existing in GK rat islets and explore the role of SC in the progression of islet fibrosis. Methods Masson staining and immunofluorescence staining assay （α-SMA/insulin） were performed using frozen sections of GK/Wistar rat pancreas. Isolating PSC by outgrowth method, and immunofluorescence staining assay for α-SMA, Vimentin, Fibronectin and Col type Ⅰ/Ⅲ were performed. Results The α-SMA positive cells increased and beta cell mass decreased in the fibrotic area in GK rat islet. Large number of SC grew out from GK rat islets, and these cells expressed α-SMA,Vimentin,FN and Col Ⅰ/Ⅲ. Conclusion The activated PSC present in fibrotic islets of GK rats and they may play an role in T2DM islet fibrosis.

关 键 词：糖尿病 2型 胰腺星状细胞 胰岛纤维化 GK大鼠 

分 类 号：R576[医药卫生—消化系统]