模拟高原训练对大鼠心肌线粒体的影响及小麦肽的干预作用  

Effect of Simulated Altitude Training on the Myocardial Mitochondria in Rats and Intervention of Wheat Peptide

作　　者：金其贯[1] 金爱娜[1] 潘兴昌[2] 汪飞[1] 刘霞[1] 蔡木易[2] 

机构地区：[1]扬州大学体育学院,江苏扬州225009 [2]中国食品发酵工业研究院,北京100027

出　　处：《体育科学》2013年第9期64-69,共6页China Sport Science

基　　金：中国食品发酵工业研究院横向基金课题

摘　　要：目的:探讨小麦肽的补充对模拟高原训练大鼠心肌线粒体结构和功能的干预作用及其机制。方法:采用交互设计的实验方案对SD大鼠进行模拟低氧(3 000m)和/或运动训练,并在每次模拟高原训练后补充小麦肽,9周后,观察心肌线粒体的超微结构,并检测大鼠心肌线粒体内ATPase、SOD活性和CCO、MDA含量。结果:1)长期的运动训练可使心肌线粒体出现轻微损伤,且心肌线粒体ATPase活性显著下降,MDA含量显著增加,CCO含量和SOD活性虽有所降低,但无显著性差异。低氧暴露可使心肌线粒体产生严重的损伤,且心肌线粒体ATPase、SOD活性和CCO含量均显著降低,MDA含量显著增加;低氧联合运动训练可使心肌线粒体的损伤更加严重,且对进一步降低ATPase活性和升高MDA含量具有显著的交互作用,但对进一步降低CCO含量和SOD活性无显著的交互作用。2)与HE组相比,HEW组心肌线粒体的损伤显著减轻,且ATPase活性和CCO含量均显著增加,MDA含量显著降低,SOD活性有所升高,但无显著性差异。结论:1)长期的高原训练能引起心肌线粒体抗氧化能力下降,氧自由基生成增多,从而心肌线粒体结构和功能发生病理性损伤。但在低氧和运动训练两个因素中,低氧对心肌线粒体结构和功能的影响占有主导地位。2)补充小麦肽可提高高原训练大鼠心肌线粒体抗氧化能力,减少氧自由基的生成,对减轻高原训练大鼠心肌线粒体的损伤,维持线粒体的正常形态结构和功能有非常重要的作用。Objective：To explore the intervention effect of wheat peptide supplement on myocar- dial mitochondrial structure and function in simulated altitude training rat and its mechanism. Methods：Used the experimental scheme of interaction design, SD rats were submitted simula- ted hypoxia （3 000 m） and / or exercise training, wheat peptides was supplement after simula- ted altitude training in every time, after 9 weeks, myocardial mitochondria ultrastructure was observed, and myocardial mitochondria ATPase, SOD activity, MDA and CCO content were measured. Results： 1） the long-term exercise training could cause slight damage of myocardial mitochondria, and mitochondrial ATPase activity decreased, MDA content increased significant- ly, CCO content and SOD activity decreased, but no significant difference. Hypoxic exposure could cause serious injury of myocardial mitochondria, and mitochondrial ATPase, SOD activity and CCO content were significantly decreased, MDA content increased significantly. Hypoxia combined with exercise training could make the myocardial mitochondria injury more serious, and it had significant interaction to further reduce ATPase activity and increase MDA content, but no significant interaction to further reduce CCO content and SOD activity. 2 ） Compared with group HE, myocardial mitochondrial injury in group HEW significantly reduced, and AT- Pase activity and CCO content were significantly increased, MDA content decreased and SOD activity increased, but there were no significant difference. Conclusion ： 1 ） Long- term altitude training can decrease antioxidant capacity of myocardial mitochondria, increase oxygen free radi- cals, which induce pathological damage of myocardial mitochondria structure and function. But in the two factors of hypoxia and exercise training, the effects of hypoxia on myocardial mito- chondrial structure and function occupies the dominant position. 2） Wheat peptide supplement can improve myocardial mitochondrial antioxidant ability of simulated altitude

关 键 词：小麦肽 高原训练 心肌线粒体 超微结构 ATPASE CCO 氧自由 鼠 动物实验 

分 类 号：G804.7[文化科学—运动人体科学]