一株不产生孢子的盐生海芦笋内生真菌鉴定  

Identification of Sterile Endophytic Fungus Isolated from Salicornia bigelovii

作　　者：王晓敏[1] 王惠[1] 刘天行[1] 辛志宏[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品科学》2013年第17期146-149,共4页Food Science

基　　金：国家自然科学基金面上项目(31071586);2011年国家大学生创新训练计划项目(111030739);江苏高校优势学科建设工程资助项目

摘　　要：对分离自盐生海芦笋的一株代号为Sal54的内生嗜盐真菌进行核糖体18S rDNA及ITS序列克隆测定,获得长度为1715bp的18SrDNA序列和长度为544bp的ITS序列,其ITS序列在GenBank的登录号为JX292134。获得的基因序列在NCBI数据库上进行同源性比对,构建18S rDNA和ITS系统发育树,并进行系统发育分析,将该菌鉴定为甜菜蛇眼茎点菌Phoma betae。The sequences of 18S rDNA and ITS gene of a fungal strain named Sal54 isolated from Salicornia bigelovii were cloned and sequenced, and a 18S rDNA gene sequence of 1715 bp in length and an ITS gene sequence of 544 bp in length were obtained. The accession number of the ITS gene sequence in GenBank was JX292134. Homology analysis was conducted. A phylogenetic tree was constructed by using these sequences and the neighbor-joining method. Phylogenetic analysis revealed that the isolated strain was identified as Phoma betae.

关 键 词：不产孢真菌 鉴定 18S RDNA基因 ITS基因 系统发育分析 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]