鱿鱼墨黑色素铁对缺铁性贫血大鼠造血调控因子的影响  

Effect of Squid Ink Melanin Fe on Hematopoietic Modulators in Rats with Iron Deficiency Anemia

作　　者：柳东[1] 王静凤[1] 张小哲[1] 龙腾腾[1] 王玉明[1] 薛长湖[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《食品科学》2013年第17期317-322,共6页Food Science

基　　金：国家海洋局海洋公益性行业科研专项(201105029);"泰山学者"建设工程专项

摘　　要：目的:探究鱿鱼墨黑色素铁对缺铁性贫血(IDA)模型大鼠造血调控因子的影响。方法:采用低铁饲料喂养辅以尾静脉定期放血建立IDA模型,用鱿鱼墨黑色素铁灌胃30d,观察其对IDA大鼠外周血红细胞(RBC)数、血红蛋白(Hb)含量、红细胞压积(HCT)、红细胞平均体积(MCV)和血清促红细胞生成素(EPO)含量的影响;分别制备大鼠肺条件培养液(LCM)、脾条件培养液(SCM)和腹腔巨噬细胞条件培养液(PMΦCM),采用MTT法检测其对正常大鼠骨髓造血细胞增殖的影响;采用单层半固体培养法检测其对粒单系祖细胞(CFU-GM)、红系祖细胞(CFU-E)和巨核系祖细胞(CFU-Meg)集落形成的影响;采用RT-PCR技术检测IDA大鼠骨髓细胞粒/单核细胞集落刺激因子(GM-CSF)、促红细胞生成素受体(EPOR)和血小板生成素(TPO)mRNA的表达。结果:鱿鱼墨黑色素铁能提高IDA大鼠RBC、Hb、HCT、MCV值,增加血清EPO含量;经鱿鱼墨黑色素铁体内诱导所制备的LCM、SCM条件培养液可促进正常大鼠骨髓细胞增殖,增加CFU-GM、CFU-E的集落形成;鱿鱼墨黑色素铁上调骨髓细胞GM-CSF和EPOR mRNA的表达。结论:鱿鱼墨黑色素铁可能诱导机体产生GM-CSF、EPO等造血细胞因子,促进粒单系、红系造血细胞的增殖分化。Objective： To investigate the effect of squid ink melanin-Fe on hematopoietic modulators in rats. Methods： Iron deficiency anemia （IDA） model rats were established by feeding with iron-deprived food and bleeding via tail vein. IDA rats were administered with squid ink melanin-Fe at different dosages for 30 days. Selected indicators such as red blood cells （RBC）, hemoglobin （Hb）, hematocrit （HCT）, mean corpuscular volume （MCV） and serum erythropoietin contents were monitored. Lung conditioned medium （LCM）, spleen cell conditioned medium （SCM） and abdominal cavity macrophage treated with squid ink melanin-Fe were prepared. The effects of the different conditional media on the proliferation of bone marrow cells and the formation of hematopoietic progenitor cells colonies including CFU-GM, CFU-E and CFU-Meg were evaluated. The mRNA expression of GM-CSF, EPOR and TPO were determined by semi-quantity RT-PCR. Results： Compared with the model group, RBC, Hb, HCT, MCV and serum erythropoietin contents in squid ink melanin-Fe group revealed a significant increase. The squid ink melanin-Fe could significantly promote the proliferation of bone marrow cells and the colony formation of CFU-GM and CFU-E in normal rats, but each colony showed various features. The squid ink melanin-Fe could increase the mRNA expression of GM-CSF and EPOR in bone marrow cells. Conclusion： The squid ink melanin-Fe can promote the formation of hematopoietic progenitor cells and the differentiation of granulocyte-macrophage and erythroid line by up-regulating hemopoietic growth factor in IDA rats.

关 键 词：缺铁性贫血 鱿鱼墨黑色素铁 粒 单核细胞集落刺激因子(GM-CSF) 促红细胞生成素受体(EPOR) 血小板生成素(TPO) 

分 类 号：R331.22[医药卫生—人体生理学]