BK病毒与肾癌的关系  

Correlation between BK virus infection and renal carcinoma

作　　者：贺剑涛[1] 耿江[1] 王波[1] 顾闻宇[1] 郑军华[1] 

机构地区：[1]同济大学附属第十人民医院泌尿外科,上海200072

出　　处：《上海医学》2013年第7期616-619,I0002,共5页Shanghai Medical Journal

基　　金：国家自然科学基金(30972984);上海市市级医院新兴前沿技术联合攻关项目(SHDC12010104);上海市卫生局新百人计划(XBR2011021)

摘　　要：目的探讨BK病毒(BKV)与肾癌发生的关系。方法收集65例肾癌患者(肾癌组)的血液、尿液、肾癌组织和癌旁肾脏组织标本,20例肾移植术后患者(肾移植组)的血液、尿液标本;10例外伤后行肾切除患者(正常组)的血液、尿液和肾脏组织标本。采用实时定量聚合酶链反应(RT-PCR)检测BKV DNA含量。结果肾癌组中有1例患者的血液、尿液、肾癌组织和癌旁肾脏组织中的BKV DNA含量为10 237.3、7 629.3、14 456.4、12 873.6拷贝/mL,显著高于其他患者,剔除该例患者,肾癌组织和癌旁肾脏组织的BKV DNA含量分别为(864.6±413.5)、(775.5±416.3)拷贝/mL,两者间差异无统计学意义(P>0.05)。肾癌组的血液、尿液BKV DNA含量为(511.1±269.2)、(369.3±195.5)拷贝/mL,有高于正常组的(325.7±119.9)、(178.1±93.5)拷贝/mL的趋势,但差异无统计学意义(P>0.05)。肾移植组的血液、尿液中BKV DNA含量分别为(4.3±1.7)×107、(3.2±1.5)×107拷贝/mL,均显著高于肾癌组和正常组(P值均<0.05)。结论肾移植患者、肾癌患者及正常人群中普遍存在BKV感染,并可引起肾移植患者有效感染,肾癌患者和正常人群均为隐性感染,其与肾癌的发生、发展可能无明显关系。Objective To explore the correlation between BK virus（BKV）and renal carcinoma.Methods We collected the blood,urine,renal tumor and adjacent normal tissues from 65renal carcinoma patients（renal carcinoma group）,blood and urine samples from 20patients after renal transplant（renal transplant group）,and blood,urine and renal tissues from 10patients undergoing nephrectomy after trauma（normal control group）.The concentration of BKV DNA in the specimens was detected by quantitative real-time polymerase chain reaction（RT-PCR）.Results The BKV DNA contents in the blood,urine,tumor tissues and adjacent normal tissues in 1sample in the renal carcinoma group was 10 237.3,7 629.3,14 456.4and 12 873.6copies/mL,respectively,which was obviously higher than other samples and excluded from the study.BKV DNA contents were not significantly different between renal tumor tissues and adjacent normal tissues（[864.6±413.5]copies/mL vs.[775.5±416.3]copies/mL,P 0.05）.There were no significant differences in the BKV DNA contents in the blood and urine between renal carcinoma group and normal control group（blood：[511.1±269.2]copies/mL vs.[325.7±119.9]copies/mL,urine：[369.3±195.5]copies/mL vs.[178.1±93.5]copies/mL,both P 0.05）.BKV DNA contents in the blood and urine in the renal transplant group was（4.3±1.7）×107 and（3.2± 1.5）×107 copies/mL,which were significantly higher than those in the renal carcinoma group and normal control group（all P0.05）.Conclusion The infection of BKV is widespread in renal transplant patients,renal carcinoma patients and normal persons,while BKV can result in effective infection in renal transplant patients.BKV infection is latent infection in both renal tumor patients and normal persons.There is no obvious relationship between BKV and the occurrence and development of renal carcinoma.

关 键 词：BK病毒 肾癌 实时定量聚合酶链反应 

分 类 号：R737.11[医药卫生—肿瘤]