肾细胞癌患者静脉血中人类斯钙素-1的表达  

Human stanniocalcin-1 in venous blood of renal cell carcinoma patients

作　　者：罗俊[1] 刘敏[1] 车建平[1] 许云飞[1] 彭波[1] 郑军华[1] 

机构地区：[1]同济大学附属第十人民医院泌尿外科,上海200072

出　　处：《上海医学》2013年第7期620-622,I0002,共4页Shanghai Medical Journal

基　　金：国家自然科学基金(30972984);上海市市级医院新兴前沿技术联合攻关项目(HDC12010104);上海市卫生局新百人计划(XBR2011021)

摘　　要：目的探讨人类斯钙素-1（hSTC-1）基因在肾细胞癌患者静脉血中的表达及其与肿瘤恶性程度的关系,为肾细胞癌的诊断提供一种新的肿瘤标志物。方法采用反转录聚合酶链反应检测25例肾细胞癌患者（肿瘤组）和25名非肿瘤人群（包括5名妊娠期妇女,对照组）的静脉血hSTC-1mRNA,并将肾细胞癌患者静脉血hSTC-1的表达结果与临床病理资料进行比较。同时采用受试者工作特征曲线（ROC曲线）进行灵敏度和特异度的综合评价。结果肿瘤组的静脉血hSTC-1基因表达总阳性率为56%（14/25）,不同性别、年龄、病理分型间的hSTC-1基因表达阳性率的差异均无统计学意义（P值均〉0.05）,肾细胞癌Ⅲ期和Ⅳ期患者的表达阳性率显著高于Ⅰ期和Ⅱ期患者（P=0.007）,伴有远处淋巴结转移者的表达阳性率显著高于无远处淋巴结转移者（P=0.042）。对照组中无1例患者的静脉血hSTC-1基因表达阳性。ROC曲线下面积为0.784,95%可信区间为0.646~0.914（P〈0.01）。结论静脉血hSTC-1基因对肾细胞癌可能有较高的诊断价值,并有望成为诊断肾细胞癌和判断其恶性程度的新的肿瘤标志物。Objective To explore the expression of human stanniocalcin-1（hSTC-1）in venous blood of patients with renal cell carcinoma and its relationship with tumor grade,so as to provide a novel tumor marker for the diagnosis of renal cell carcinoma.Methods The expression of hSTC-1mRNA in venous blood of 25patients with renal cell carcinoma and 25people without tumor（including 5pregnant women）was determined by reverse transcription polymerase chain reaction（RT-PCR）.The results were compared with clinical pathological data.Comprehensive evaluation of sensitivity and specificity was conducted by receiver operating characteristic（ROC） curve.Results Among the 25patients with renal cell carcinoma,14（56%）showed positive expression of hSTC-1.There was no significant difference in hSTC-1 mRNA expression among different genders,ages or pathological types（all P0.05）,while there was statistical difference in hSTC-1mRNA expression between stageⅠ-Ⅱ and stageⅢ-Ⅳ of renal cell carcinoma（P=0.007）,and between the patients with and without distant lymph node metastasis（P=0.042）.hSTC-1was not found in the venousl blood of people without tumor.ROC area under curve was 0.784,and 95% confidence interval was 0.646-0.914（P0.01）.Conclusion The mRNA level of hSTC-1in venous blood has a high diagnostic value for renal cell carcinoma.It may be a novel tumor marker for diagnosis and staging of renal cell carcinoma.

关 键 词：斯钙素-1 肾细胞癌 肿瘤标志物 

分 类 号：R737.11[医药卫生—肿瘤]