5-羟色胺与褪黑素在母婴分离致腹泻型肠易激综合征模型大鼠中的表达  

Expression of 5-HT and MT in IBS-D rats caused by maternal separation

作　　者：石卿[1,2] 赵云[1] 梅竹松[1] 刘伟丽[1] 杲修杰[1] 姚林[2] 钱令嘉[1] 

机构地区：[1]军事医学科学院基础医学研究所,全军军事认知与心理卫生研究中心,北京100850 [2]河北联合大学公共卫生学院,河北唐山063000

出　　处：《军事医学》2013年第8期604-608,共5页Military Medical Sciences

基　　金：国家973计划资助项目(2011CB505106)

摘　　要：目的探讨母婴分离致腹泻型肠易激综合征(IBS-D)大鼠血浆中5-羟色胺(5-HT)及褪黑素(MT)的表达。方法建立新生期母婴分离致IBS-D大鼠模型,采用糖水偏嗜实验评价模型动物精神行为学的改变;对动物排便情况进行观察与计数分析,并进行结直肠扩张(CRD)和腹壁撤退反射(AWR)实验,研究IBS-D模型大鼠肠道感觉运动功能的变化;采用ELISA试剂盒检测大鼠血浆5-HT和MT含量。结果与对照组相比,IBS-D模型大鼠糖水偏嗜度显著降低,粪便颗粒含水量增多且数量增加,大鼠腹部抬起和弓背的容量阈值显著降低,血浆中5-HT及MT的含量较对照组显著升高。结论 IBS可导致大鼠快感消失以及肠道运动及感觉功能异常,这种改变可能与血浆中的5-HT和MT的表达升高有关。Objective To explore the expression of serotonin（5-HT） and melatonin（MT） in irritable bowel syndrome with diarrhea（IBS-D） rats caused by maternal separation（MS）.Methods An IBS-D rat model was established by MS and the rats＇ defecation was observed to identify the changes in mental behavior.Then sucrose preference test,colono-rectal distension（CRD） and abdominal withdrawl reflex（AWR） tests were conducted to assess intestinal functions.Blood samples were taken and plasma 5-HT and MT were determined using ELISA kit.Results Compared with control group,the sucrose preference degree was significantly reduced in IBS-D model group.The number of abdominal lift and bucking was also reduced.Feces were increased both in quantity and water content.The expression of plasma 5-HT and MT in IBS-D rats was increased significantly.Conclusion IBS-D rats undergo anhedonia and significant changes in intestinal functions,which may be related to the expression change in 5-HT and MT in blood plasma.

关 键 词：母婴分离 肠易激综合征 腹泻 血清素 褪黑素 

分 类 号：R574.62[医药卫生—消化系统]