川芎嗪抑制晶体上皮细胞增殖的实验研究  

Study on the inhibitory effects of tetramethylpyrazine on proliferation of bovine lens epithelial cells

作　　者：陈义[1] 金海霞[1] 

机构地区：[1]安徽中医药大学第一附属医院眼科,安徽合肥230038

出　　处：《中华疾病控制杂志》2013年第9期803-805,共3页Chinese Journal of Disease Control & Prevention

基　　金：安徽中医药大学青年基金(2006qn016)

摘　　要：目的探讨川芎嗪(tetramethylpyrazine,TMP)对重组人表皮生长因子(recombinant human epidermal growth factor,rhEGF)诱导的牛晶状体上皮细胞(bovine lens epithelial cell,BLEC)增殖的抑制作用。方法采用BLEC进行体外原代及传代培养,利用rhEGF诱导BLEC增殖,同时用低、中、高三种不同浓度的TMP作用处于增殖状态下的BLEC。采用MTS法测定细胞增殖情况。通过RT-PCR方法检测正常对照组、增殖对照组和低、中、高浓度TMP组的Bcl-2的基因表达水平。结果 MTS检测发现,TMP以剂量依赖方式抑制rhEGF诱导的BLEC增殖。RT-PCR发现增殖对照组的Bcl-2表达增高,TMP可以下调Bcl-2的表达水平,两者差异有统计学意义。结论 TMP能有效抑制rhEGF诱导的BLEC增殖。Objective To detect the inhibitory effects of tetramethylpyrazine （TMP） on proliferation of bovine lens epithelial cells （BLEC） induced by recombinant human epidermal growth factor （rhEGF）. Methods BLEC were primari- ly cultured and sub-cultured. BLEC which have been induced to proliferate by rhEGF were incubated with low, middle and high concentration of TMP. The activity of BLEC was detected by MTS, Bcl-2 mRNA expression was detected in the normal control group, control group and low, middle, high concentration of TMP groups by RT-PCR. Results Proliferation of BLEC induced by rhEGF was inhibited by TMP with dose-effect manner. The expression of Bcl-2 mRNA was significantly increased in control group, and the expression of Bcl-2 was reduced in groups of low, middle and high concentration of TMP. Conclusions TMP can effectively inhibit the proliferation of BLEC induced by rhEGF.

关 键 词：川芎嗪 上皮细胞 细胞增殖 

分 类 号：R776.1[医药卫生—眼科] R287[医药卫生—临床医学]