MVA与MEP途径抑制剂对人参皂苷合成能力的影响研究  

Research on Ginsenoside Synthesis Capability by Utilizing MVA and MEP Pathway Inhibitors

作　　者：钱延春[1] 刘丽[1] 曹豪杰[1] 吴明[1] 庄园[1] 曲庆玲[1] 赵寿经[1] 

机构地区：[1]吉林大学生物与农业工程学院,长春130022

出　　处：《特产研究》2013年第3期44-50,共7页Special Wild Economic Animal and Plant Research

基　　金：国家自然科学基金(30970259;31270337);"十二五"国家科技计划(2013AA102604-3);高等学校博士点专项科研基金(20120061110038)

摘　　要：通过MVA与MEP途径的抑制剂洛伐他汀和膦胺霉素不同浓度及作用时间对人参发根生长影响的研究,确定洛伐他汀和膦胺霉素对人参总皂苷合成的最高抑制率发生在接种后第3天,分别为57.94%和42.17%。通过对人参发根中单体皂苷Rg1、Re和Rb1含量的HPLC测定发现,洛伐他汀(50μmol/L)对上述单体皂苷合成的最高抑制率依次为50.38%、66.66%和40.11%;膦胺霉素(15μmol/L)对上述单体皂苷的最高抑制率依次为38.53%、38.85%和19.46%,与抑制剂对人参总皂苷含量的影响规律基本一致。推断人参皂苷的生物合成可能同时存在MVA和MEP途径,而MVA途径在人参皂苷的生物合成中可能起主导作用。In different concentration and feeding time of inhibitor for ginseng hairy root growth study ,we found that lovastatin and fosmidomycin did not inhibit the growth of hairy root when concentrations were lower than 50μmol/L and 15μmol/L ,and the most suitable feeding time was 3 days .Under the above-mentioned conditions ,the maximum inhibition rate of lovastatin and fosmidomycin on the biosynthesis of total saponin were 57.94% and 42.17% respectively .Experimental investigation on content of monomer saponin Rg1、Re and Rb1 in ginseng hairy root indi-cated that the maximum inhibition rate of lovastatin on Rg1、Re and Rb1 were 50.38% ,66.66% ,40.11% respectively ,and the maximum inhi-bition rate of fosmidomycin were 38.53% ,38.85% ,19.46% respectively ,which coincided with the results of variation of total saponin .There-fore ,we concluded that both MVA pathway and MEP pathway existed in the biosynthesis of ginsenosides ,and among them MVA pathway played the dominant role .

关 键 词：人参皂苷 洛伐他汀 膦胺霉素 MVA途径 MEP途径 

分 类 号：TQ414.6[化学工程]