胃腺癌中胎盘型谷胱甘肽转移酶π、P-糖蛋白、拓扑异构酶Ⅱ及胸苷酸合成酶的表达及其临床意义  

Expression of glutathione-S-transferase-α, P-glycoprotein, topoisomerase Ⅱ and thymidylate synthase in gastric adenocarcinoma tissues and their clinical significances

作　　者：杨东杰[1] 姚宏[2] 茅青[1] 冯晓星[1] 谢碧琛[1] 

机构地区：[1]南通大学第三附属医院病理科,无锡214041 [2]太原市人民医院病理科

出　　处：《肿瘤研究与临床》2013年第9期595-598,共4页Cancer Research and Clinic

摘　　要：目的探讨胎盘型谷胱甘肽转移酶π（GST-π）、P-糖蛋白（P—gP）、拓扑异构酶Ⅱ（TopoⅡ）及胸苷酸合成酶（TS）在胃腺癌组织中的表达特点及其临床意义。方法应用免疫组织化学Envision法联合检测GST-π、P—gP、TopoII及TS在64例胃腺癌组织和12例正常胃黏膜组织中的表达，并结合其临床资料进行综合分析。结果胃腺癌组织中GST-π、P-gP、TopoⅡ及TS的表达阳性率分别为76．6％（49／64）、64．1％（41／64）、84．4％（50／64）和53．1％（34／64），均高于其在正常胃黏膜组织中的8．3％（1／12）、83％（1／12）、16．7％（2／12）、0，其差异均有统计学意义（P〈0．01）；它们在胃腺癌组织中的表达与患者的性别、年龄、肿瘤大小、临床分期、侵袭深度及淋巴结转移情况均无关（P〉0．05），而与肿瘤的分化程度有关（P〈0．05），GST-π、P-gp及Ts在高、中分化腺癌中的表达均高于低分化腺癌，而TopoⅡ在高、中分化腺癌中的表达低于低分化腺癌（P〈0．05）。结论GST-π、P-gP、TopoⅡ及Ts在胃腺癌中过表达与胃腺癌的多药耐药相关。联合检测GST-π、P-gP、Topo1及Ts在胃腺癌组织中的表达，对化疗药物的选择及预后的判断有一定的临床指导意义。Objective To explore the expression of glutathione-S-transferase-π （GST-π）, P-glycoprotein （P-gp）, topoisomerase II （Topo ]I ） and thymidylate synthase （TS） in gastric adenocarcinoma tissues and the clinical significance. Methods Envision method of immunohistochemistry was used to detect the expression of GST-π, P-gp, Topo II and TS in sample of 64 gastric adenocarcinoma tissues and 12 normal gastric mucosas, and their corresponding clinical data were comprehensive analyzed. Results The expression positive rates of GST-π, P-gp, Topo lI and TS were respectively 76.6 %（49/64）, 64.1% （41/64）, 84.4 % （50/64） and 53.1% （34/64）, that were all higher than in gastric mucosa [8.3 % （1/12）, 8,3 %（1/12）, 16.7 % （2/12）, 0], the differences were statistically significant respectively （P 〈 0.01）. Their positive expression rates were closely relevant to the degree of differentiation （P 〈 0.05）, but not to the patients gender, age, tumour size, clinical staging, invasive depth and lymph node metastasis （P 〉 0.05）. The expressions of GST-π, P-gp and TS in high and middle differentiation adenocarcinoma were higher than in low differentiation, but the expression of Topo 11 in high and middle differentiation adenocarcinoma was lower than in low differentiation （P 〈 0.05）. Conclusion GST-π, P-gp, Topo 11 and TS are over-expressed in gastric adenocarcinoma, which is related to the multidrug resistance of gastric adenocarcinoma. The joint detection of the expression of GST-π, P-gp, Topo π and TS in gastric adenocarcinoma can be looked as an important symbol for guiding its chemotherapy drug and prognosis.

关 键 词：胃肿瘤 多药耐药 谷胱甘肽转移酶盯 P-糖蛋白 拓扑异构酶Ⅱ 胸苷酸合成酶 

分 类 号：R735.2[医药卫生—肿瘤]