食管癌中PED／PEA-15mRNA及蛋白表达的临床意义  

Expression of PED/PEA-15 mRNA and protein in esophageal carcinoma

作　　者：庄晓飞[1] 张弘广[1] 王春利[1] 郭石平[1] 马炎炎[1] 解立武 

机构地区：[1]山西省肿瘤医院胸外二科,太原030013 [2]病理科

出　　处：《肿瘤研究与临床》2013年第9期599-601,共3页Cancer Research and Clinic

基　　金：山西省留学人员科研项目（115）

摘　　要：目的探讨PED／PEA．15mRNA及蛋白在食管癌组织中的表达情况及其临床意义。方法应用半定量反转录一聚合酶链反应（RT．PCR）检测50例食管癌、相应癌旁组织和正常食管组织中PED／PEA．15基因的相对表达量，并通过免疫组织化学检测PED／PEA-15蛋白的表达。结果半定量RT—PCR显示，PED／PEA．15基因在食管癌组织中均呈高表达，癌旁组织及正常食管组织呈低表达或不表达，相对表达量分别为1．14±0．49、0．594±31和0．53±0．22，PED／PEA．15mRNA在食管癌组织中表达较癌旁组织、正常食管组织升高（F=44．085，P〈0．01）。免疫组织化学结果显示，食管癌组织中PED／PEA．15蛋白阳性表达率高于正常食管组织和癌旁组织（x^2=36．967，P〈0．001；x^2=26．272，P〈0．001）。且PED／PEA．15mRNA及蛋白的表达与食管癌病理分级、临床分期有关（P〈0．05），而与发病年龄、性别、病理类型无相关性（P〉0．05）。结论PED／PEA-15mRNA及蛋白在食管癌组织中表达水平明显升高，可能在食管癌的发生及发展过程中发挥重要作用。Objective To investigate the expression of PED/PEA-15 mRNA and protein in esophageal carcinoma tissue and their clinical significanccs. Methods The expression of PED/PEA-15 mRNA was detected by RT-PCR, and the expression of PED/PEA-15 protein was measured by immunohistochemistry in 50 cases of esophageal carcinoma, 50 cases of corresponding paracarcinoma tissue,and 50 cases of corresponding normal esophageal tissue. Results The expression of PED/PEA-15 mRNA was 1.14±0.49 in esophageal carcinoma, which was significantly higher than that in para-carcinoma tissue （0.59±0.31） and normal esophageal carcinoma tissue （0.53±0.22） （F = 44.085, P 〈 0.001）. The immunohistochemistry results showed that PED/PEA-15 protein expression was significantly higher than that in para-carcinoma tissue and normal esophageal tissue （X2 = 36.967, P 〈 0.001; x^2 = 26.272, P 〈 0.001）. The expression of PED/PEA-15 mRNA and protein were significantly associated with pathological grade, clinical stage of esophageal carcinoma （P 〈 0.05）, but were not significantly correlated to the age of onset, gender, pathological types （P 〉 0.05）. Conclusion The expression of PED/PEA-15 mRNA and protein are increased in esophageal carcinoma, which may play an important role in the occurrence and development of esophageal carcinoma.

关 键 词：食管肿瘤 反转录聚合酶链反应 PED PEA-15 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤]