三重四极杆液质联用法测定花生中4种黄曲霉毒素的研究  

Simultaneousdetermination of aflatoxin B1,B2,G1 and G2 in peanuts by liquid chromatography – triple quadrupole tandem mass spectrometer

作　　者：贾玉珠[1] 贾沁一[2] 周娜[1] 白艳艳[1] 牛建军[1] 

机构地区：[1]厦门市疾病预防控制中心,厦门361021 [2]北京化工大学,北京102200

出　　处：《中国卫生检验杂志》2013年第10期2224-2226,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立LC/MS/MS法同时检测花生中黄曲霉毒素B1、B2、G1和G2的方法。方法粉碎后混匀的样品经70%甲醇提取,免疫亲和柱净化,在MRM模式下用LC/MS/MS法测定食品中黄曲霉毒素B1、B2、G1和G2含量。结果黄曲霉毒素B1、B2、G1和G2的加标回收率77%～109%,相对标准偏差为3.2%～5.7%;在0.1 ng/ml～100 ng/ml浓度范围内具有很好线性,相关系数大于0.999;方法检出限小于0.08μg/kg、定量限小于0.3μg/kg。结论该法简单、快速、灵敏和特异性强,可同时对花生中黄曲霉毒素B1、G1、G2和B2进行定性确证和定量测定。Objective Developing a method to determine 4 aflatoxins in peanuts by LC/MS/MS. Methods The samples were extracted by 70% methanol, purified with immunoaffinity column and then determined the content of aflatoxin B1, B2, G1 and G2 in food in MRM mode by LC / MS / MS method. Results The average recovery of the aflatoxins was 77% - 109% in peanuts, the relative standard deviations was 3.2% - 5.7%. The method has a good linearity in the concentration range of 0.1 ng/ml - 100 ng/ml, and the correlation coefficient is greater than 0. 999. The detection limit was less than 0.08 μg/kg, and the quantification limit was less than 0.3μg / kg. Con- clusion The method is more simple, rapid, sensitive, specific and suitable for the qualitative and quantitative de- termination of aflatoxin B1, B2, G1 and G2 in foods simultaneously.

关 键 词：免疫亲和柱 三重四极杆液质联用法 花生 黄曲霉毒素 

分 类 号：O657.63[理学—分析化学]