获得构建miRNA高表达载体的pre-miRNA及其侧翼DNA序列的生物信息学方法  

Bioinformatic methods for obtaining pre-miRNA and its flanking DNA sequence for constructing miRNA high-expression vector

作　　者：李嘉熙[1,2] 宋刘梅[1,2] 王美晨[1,2] 蓝茜[1] 李玥[1] 刘莉[1] 王璇[1] 韩燕[1] 李冬民[1] 

机构地区：[1]西安交通大学医学部生物化学与分子生物学系,西安710061 [2]西安交通大学医学部临床医学七年制,西安710061

出　　处：《中国医学教育技术》2013年第5期546-549,共4页China Medical Education Technology

基　　金：国家自然科学基金(81370952);中央高校基本科研业务费专项基金项目(xjj20100031);陕西省科技计划项目(2013K21-22-03);西安交通大学本科生科研训练和实践创新基金项目(22012185及2013273);国家级大学生创新训练项目(201310698055)资助

摘　　要：近年研究发现pre-miRNA发夹结构两侧的序列对miRNA的成熟至关重要。文章主要介绍了利用"miRBase"和"Ensembl"数据库获得构建microRNA高表达载体的pre-miRNA及其侧翼DNA序列的生物信息学方法:即先通过"miRBase"数据库获得pre-miRNA在"Ensembl"数据库的位置链接;然后在"Ensembl"数据库内获得pre-miRNA在基因组上的位置及碱基序列;最后在pre-miRNA序列导出窗口5'和3'侧翼序列输入框内填写所需侧翼序列的长度,进而获得pre-miRNA及其侧翼的DNA序列。In recent years , studies have been reported that the both-side flanking sequence of the hairpin structure of pre-miRNA is crucial for miRNA maturation .This article mainly introduces the bioinformatic methods for obtaining pre-miRNA and its flanking DNA sequence for constructing miRNA high-expression vector by miRBase and Ensembl Databases .First, location links in Ensembl Database of pre-miRNA were obtained with miRBase Database .Then, the genomic location and base sequence were obtained from Ensembl Database .Finally, according to the required length of 5 ＇and 3＇flanking sequences , the DNA sequence of pre-miRNA and its flanking were received .

关 键 词：“miRBase”数据库 “Ensembl”数据库 成熟miRNA miRNA前体(precursormicroRNA pre-miRNA) pre-miRNA侧翼 

分 类 号：G434[文化科学—教育学]