β-连环素对人羊膜间充质干细胞增殖的影响  

Influence of β-catenin on the proliferation of human AMSC

作　　者：张一折[1] 芦俊萍[1] 张东丽[1] 梁硕[1] 迟连凯[1] 赵永辉[1] 王满仓[1] 何涛[1] 乔露华[1] 田毅[2] 杨波[2] 马轩[1] 关方霞[1] 

机构地区：[1]郑州大学生物工程系,郑州450001 [2]郑州大学第一附属医院神经外科,郑州450052

出　　处：《郑州大学学报（医学版）》2013年第5期602-605,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目81071008;河南省杰出青年基金资助项目114100510005;河南省教育厅科学技术研究重点项目13A180656

摘　　要：目的:探讨β-连环素(β-catenin)对人羊膜间充质干细胞(AMSC)增殖的影响。方法:将β-catenin-绿色荧光蛋白(β-catenin-GFP)重组腺病毒扩增后,转染人AMSC,在荧光倒置显微镜下观察转染效率。采用CKK-8法测定AMSC的细胞生长状况。结果:荧光观察证实β-catenin-GFP融合蛋白在AMSC细胞中获得有效表达,β-catenin稳定高表达的AMSCs增殖率明显高于正常的AMSCs,分别为50.0%、82.5%、92.6%、169.1%和128.2%。结论:在AMSC细胞中成功表达了β-catenin-GFP融合蛋白,β-catenin高表达能有效地刺激AMSC的增殖。Aim：To observe the influence of β-catenin on the proliferation of human amniotic membrane derived mes-enchymal stem cells （ AMSC） .Methods：The adenovirus carrying human β-catenin-GFP was amplified and then transfected into human AMSC , followed by observation of the cells by fluorescent microscope .The life span was detected by CKK-8 methods.Results：The expression of β-catenin-GFP fusion protein was confirmed by immunofluorescence studies .Com-pared with the control normal cell , the proliferation rate of AMSCs whith high β-catenin expression was obviously higher , respectively, 50.0%, 82.5%, 92.6%, 169.1%and 128.2%.Conclusion： The fusion protein can be successfully ex-pressed in human AMSC .The high expression of β-catenin can effectively stimulate AMSC proliferation .

关 键 词：Β-连环素 重组腺病毒 羊膜间充质干细胞 

分 类 号：R739.41[医药卫生—肿瘤]