大尺寸SiO2大孔材料固定化漆酶  

Immobilization of Laccase on Large-Sized SiO_2 Macroporous Materials

作　　者：张群[1] 张育淇[1] 刘晓贞[1] 梁云霄[1] 张瑞丰[1] 龙能兵[1] 

机构地区：[1]新型功能材料及其制备科学国家重点实验室培育基地宁波大学材料科学与化学工程学院,宁波315211

出　　处：《无机化学学报》2013年第10期2065-2070,共6页Chinese Journal of Inorganic Chemistry

基　　金：浙江省自然科学基金(No.LY12B01004);宁波市社会发展项目(No.2011C50052);宁波大学王宽诚幸福基金(No.XKL072);先进能源材料化学教育部重点实验室(南开大学)开放课题(No.KLAEMC-OP201201)资助项目

摘　　要：以表面固定Cu2+的改性大尺寸SiO2大孔材料作为载体,考察了时间、pH和给酶量对漆酶固定化效果的影响,并对固定化漆酶的活性和稳定性进行了研究。结果表明:5 h时吸附达到平衡,pH为4.5、漆酶与载体比例为5 mg·g-1时固定化效果最好,酶活回收率可达到100.4%;固定化漆酶的最适pH和最适温度较游离漆酶的均有升高且范围变宽,固定化后,漆酶的pH稳定性和热稳定性都得到显著提高;固定化漆酶的K m值略高于游离漆酶的;固定化漆酶具有良好的操作稳定性,与底物反应反复操作10批次后剩余酶活为72.7%。A large-sized SiO2 macroporous material modified by fixing Cu2＋ on its surface was employed as the support for the immobilization of laecase. The effects of immobilization time, pH value and the amount of enzyme provided on the immobilization were investigated, and the activity and stability of the immobilized enzyme were also studied. The results show that the adsorption equilibrium is reached after about 5 hours. The optimum conditions for immobilization of lacease were at the immobilization pH of 4.5 and the ratio of enzyme to support of 5 mg＇g-1, and in this case, the activity recovery of the immobilized laeease is 100.4%. Comparing with the free laecase, both the optimum working pH and temperature of the immobilized lacease are higher and the ranges are wider. The experiments show that the immobilization improves the pH stability and the thermal stability of laccase significantly. The Km of the immobilized laccase is higher than the free laccase＇ s. The immobilized laccase has good operational stability, the remained activity is 72.7% after 10 times of reaction with the substrate.

关 键 词：漆酶 固定化 大尺寸 SiO2大孔材料 

分 类 号：Q814.2[生物学—生物工程]