分泌型簇集素蛋白在肺癌细胞株中生物学行为的研究  

The study of secretory clusterin on the biological behaviors of non-small cell lung cancer cells

作　　者：马晓[1] 罗晓莹[2] 李宗海[2] 李鹤成[1] 

机构地区：[1]复旦大学附属肿瘤医院胸外科,复旦大学上海医学院肿瘤学系,上海200032 [2]上海交通大学医学院附属仁济医院上海市肿瘤研究所,上海200032

出　　处：《中国癌症杂志》2013年第9期697-702,共6页China Oncology

基　　金：国家自然科学基金项目(No:81272608);上海市科技启明星跟踪计划(No:11QH1400600)

摘　　要：背景与目的:簇集素蛋白(clusterin,CLU)在肺癌的发生、发展中发挥重要作用。本研究用分泌型CLU体外处理肺癌细胞株,旨在探讨分泌型CLU对肺癌细胞株的影响。方法:用真核表达纯化的分泌型CLU处理非小细胞肺癌细胞株H460、A549,采用Boyden小室试验检测细胞的迁移能力,用CCK8检测细胞的增殖能力,利用芯片技术分析CLU处理H460细胞的microRNA表达谱,然后采用实时荧光定量PCR检测分泌型CLU处理H460细胞中microRNA的表达,初步分析分泌型CLU执行肿瘤生物学功能的microRNA相关分子机制。结果:分泌型CLU处理组H460迁移的细胞是BSA处理组的3.10倍(P<0.000 1);分泌型CLU处理组A549迁移的细胞是BSA处理组的3.40倍(P<0.000 1);分泌型CLU抑制了肺癌细胞株H460/A549的生长(P<0.000 1);利用芯片技术发现microRNA-302b-3p、microRNA-23a-5p和microRNA-101-5p高表达。结论:在非小细胞肺癌细胞株H460/A549中,真核表达的分泌型CLU促进了细胞迁移、抑制了细胞生长,并且能引起microRNA表达谱的改变,本研究为探索分泌型CLU在非小细胞肺癌中的作用提供了重要的实验依据。Background and purpose： Primary study showed that clusterin was associated with tumorigenicity. The goal of the study is to investigate the role of secretory clusterin in non-small cell lung cancer cell A549/H460. Methods： The lung cancer cell A549/H460 was treated with purified secretory clusterin, the Boyden Chamber migration assay was used to detected the migration of the lung cancer cell; the CCK8 assay was used to detected the growth of the ceils; microRNA expression spectrum in H460 treated with secretory clusterin was analyzed, after that, we used the real-time florescence quantification detected the expression of microRNA in H460, and the biological function of microRNA molecular mechanisms of secretory clusterin was analyzed. Results： Secretory clusterin promoted the migration in A549/H460 （P〈0.000 1）; Secretory clusterin inhibited the growth in H460/A549 （P〈0.000 1）; MicroRNA-302b-3p, microRNA-23a-5p and microRNA-101-5p was overexpressed in H460 when treated with secretory clusterin. Conclusion： Secretory clusterin could promote the migration and inhibit the growth of lung cancer cell; It could change the microRNA expression spectrum as well. Our studies revealed that secretory clusterin could be used as a tool for further study, and it is a potential target in the treatment of lung cancer.

关 键 词：分泌型簇集素蛋白 非小细胞肺癌 细胞迁移 microRNA 

分 类 号：R734.2[医药卫生—肿瘤]