白花丹醌对人肝癌SK-hep-1细胞增殖及侵袭的影响  

The effects of plumbagin on proliferation and metastasis in human liver cancer SK-hep-1 cells

作　　者：曹晓淬[1] 王卉[1] 张红梅[1] 刘怡雯[1] 卢忠心[1] 

机构地区：[1]华中科技大学同济医学院附属武汉市中心医院检验科,湖北武汉430014

出　　处：《中国癌症杂志》2013年第9期721-727,共7页China Oncology

摘　　要：背景与目的:白花丹醌是中药白花丹的主要活性成分,对多种肿瘤细胞具有杀伤作用。本研究旨在观察白花丹醌对人肝癌SK-hep-1细胞增殖及侵袭的影响,并初步探讨其作用机制。方法:在体外应用MTS法、软琼脂克隆形成实验、流式细胞术及Transwell小室观察白花丹醌对细胞增殖及侵袭的影响;并通过RTPCR检测白花丹醌对p21、MMP-2及MMP-9的mRNA表达影响。结果:白花丹醌能够明显抑制肝癌SK-hep-1细胞的增殖和克隆形成,且具有剂量依赖性,其半数抑制率为22.04μmol/L。细胞周期分析显示,白花丹醌处理后S期细胞数减少,G0/G1期细胞增多;并且白花丹醌能够抑制SK-hep-1细胞的黏附和侵袭转移。RT-PCR结果显示白花丹醌能够促进p21表达,而抑制MMP-2及MMP-9的表达。结论:白花丹醌可能是通过上调p21及下调MMP-2/MMP-9的表达水平,抑制人肝癌SK-Hep-1细胞增殖和侵袭。Background and purpose： Plumbagin is the main active components of traditional Chinese medicine of plumbago zeylanica. The present studies show that plumbagin has a killing effect on tumor cells. This study aimed to investigate the function and primary mechanism of plumbagin on invasion and metastasis of human liver cancer SK-hep-1 cells. Methods： With the treatment of plumbagin in vitro, cell proliferation and adhesion of SK-hep-1 cells were detected by MTS staining, cell cycle of SK-hep-1 cells were detected by flow cytometry, the self- renewal and propagation abilities of SK-Hep-1 cells were conducted by colony formation assay, invasion in cells were performed using transwell invasion assay, and the p21 and MMP-2/9 mRNA levels were detected by real-time RT-PCR. Results： With the treatment of plumbagin, SK-Hep-1 cells proliferation was decreased with plumbagin concentration- dependency and the ICs0 value of plumbagin in SK-Hep-1 cells was 22.04 mmol/L. The colony formation ability of SK- Hep-1 cells was decreased and the percentage of cells in G0/G1 phase was increased in a dose-dependent manner, as compared to control. The cell adhesion and invasion abilities were decreased. The real-time RT-PCR showed that p21 mRNA expression was increased and the MMP-2/9 mRNA was decreased. Conclusion： Plumbagin could suppress the proliferation and invasiveness of human liver cancer SK-hep-1 cells in vitro, and these effects may be by up-regulation of p21 and down-regulation of MMP-2 and MMP-9.

关 键 词：增殖 肿瘤转移 白花丹醌 肝癌 SK-hep-1 

分 类 号：R735.7[医药卫生—肿瘤]