UNC5H3和DCC在胃癌组织中的表达与增殖的关系及其预后  

Relationship between UNC5H3,DCC,proliferation and prognosis in gastric cancer

作　　者：陈金明[1] 王沈华[1] 吴定钱[1] 戴梦昭 韩宝三[3] 

机构地区：[1]浙江大学医学院附属第二医院外科,杭州310009 [2]杭州小河湖墅社区卫生服务中心,杭州310000 [3]上海交通大学医学院附属新华医院普外科,上海200092

出　　处：《解剖学报》2013年第5期651-655,共5页Acta Anatomica Sinica

基　　金：浙江省自然科学基金资助项目(Y207543);杭州市科技局推进社会发展基金资助(20061133B16);杭州市科技局推进社会发展资助项目(20110833B41)

摘　　要：目的探讨UNC5H3和DCC在胃癌组织的表达,与临床病理特征和增殖的关系以及与患者生存和预后的关系。方法应用组织芯片和免疫组织化学技术,分析60例胃癌组织中UNC5H3、DCC和Ki-67的表达,并对其表达进行相关分析。利用图像分析软件Image-Pro Plus 6.0测量切片积分吸光度,对结果进行验证。采用Kaplan-Meier限乘法计算生存率。建立Cox回归模型,评价UNC5H3和DCC作为胃癌患者预后的独立影响因素的可行性。结果在60例胃癌组织中,UNC5H3、DCC和Ki-67的阳性表达率分别为43.3%、53.3%和60.0%。UNC5H3和DCC与淋巴转移和远处转移之间,差异有显著性(P<0.05)。UNC5H3和DCC表达与Ki-67表达相互之间无相关性(P>0.05)。用Kaplan-Meier生存曲线经Log-rank检验发现,UNC5H3的阳性表达与胃癌患者生存之间存在相关性(P<0.05)。DCC的阳性表达与胃癌患者生存之间无相关性(P>0.05)。经Cox回归分析,UNC5H3和DCC的表达与胃癌患者预后无明显相关性。结论依赖性受体UNC5H3和DCC共同参与了胃癌的发生进展,检测UNC5H3和DCC可作为反映胃癌临床病理学特点的指标,检测UNC5H3可作为胃癌患者生存期的指标,但UNC5H3和DCC的表达不是判断胃癌患者预后的独立影响因素。Objective To investigate dependence receptors UNC5H3 and DCC in gastric cancer tissues and their association with proliferation, biological features, survival, and prognosis. Methods Tissue micro-array and immunohistochemistry were used to detect expression of UNC5H3, DCC and Ki-67. Integrated absorbance of stained sections was measured by Image-Pro Plus 6.0,and the expressional analysis was also verified.The relationship between UNC5H3, DCC and Ki-67 as analyzed.Survival was evaluated by Kaplan-Meier survival curves. Cox regression analysis was performed to evaluate the possibility of UNC5H3 and DCC as independent prognostic factors for gastric cancer. Results The positive-expression rate of UNC5H3, DCC and Ki-67 in tumor tissues from 60 patients with gastric cancer were 43.3%, 53.3% and 60.0%, respectively. UNC5H3 and DCC expressions were related to lymph node metastasis and distant metastasis （P〈0.05）. No correlation between UNC5H3, DCC and K-i67 was observed （P〈0.05）. Using Kaplan-Meier survival curves and the Log-ranktest, there was correlation between UNC5H3 and survival （P〈0.05）. No correlation between DCC and survival was observed （P〉0.05）. Using Cox regression model, UNC5H3 and DCC expressions were not significantly correlated with the prognosis of gastric cancer. Conclusion UNC5H3 and DCC may be related with tumorigenesis and tumor progression. The detection for UNC5H3 and DCC may be helpful to evaluate the clinicopathological parameters. The detection for UNC5H3 may also be helpful to evaluate survival time. However, UNC5H3 and DCC may not be an independent prognostic factor for gastric cancer.

关 键 词：胃癌 UNC5H3 DCC 增殖 预后 组织芯片 免疫组织化学 人 

分 类 号：R735[医药卫生—肿瘤]