Calcineurin和TRPC6参与ET-1诱导的肺动脉平滑肌细胞增殖  

Calcineurin and TRPC6 are involved in ET-1-induced proliferation of pulmonary artery smooth muscle cells

作　　者：王小闯[1] 李满祥[2,3] 党晓燕[1] 李萍[1] 彭卓[1] 高彦霞[1] 古长维[1] 

机构地区：[1]西安交通大学医学院第二附属医院急诊医学科,陕西西安710004 [2]西安交通大学医学院第二附属医院呼吸科,陕西西安710004 [3]西安交通大学医学院第二附属医院呼吸病研究室,陕西西安710004

出　　处：《南京医科大学学报（自然科学版）》2013年第9期1191-1195,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助(81070045);卫生部部属(管)医院临床学科重点项目(111)

摘　　要：目的:探讨Calcineurin和瞬时感受器电位6(TRPC6)的相互作用以及对内皮素-1(ET-1)诱导的肺动脉平滑肌细胞增殖的影响。方法:以ET-1刺激肺动脉平滑肌细胞(PASMCs)检测Calcineurin活性以及TRPC6的表达;分别于ET-1刺激前给予Calcineurin特异性抑制剂环孢素A(CsA)和TRPC6 siRNA处理,测定Calcineurin活性、TRPC6的表达以及细胞的增殖情况;采用Calcineurin活性检测试剂盒检测其活性,Western blot和RT-PCR检测TRPC6的表达,MTT法检测PASMCs的增殖。结果:ET-1可激活原代培养的PASMCs中Calcineurin的活性,上调TRPC6的表达;CsA和TRPC6 siRNA可以分别降低TRPC6表达和Calcineurin活性;也可以抑制ET-1刺激的PASMCs增殖。结论:Calcineurin与TRPC6参与ET-1诱导的肺动脉平滑肌细胞增殖并相互影响。Objective：To explore the interaction between Calcineurin and TRPC6, and the impact of this interaction on endothelin-1（ET-1）-induced proliferation of pulmonary artery smooth muscle cells （PASMCs）. Methods：PASMCs were treated with ET-1 to stimulate their proliferation with or without prior treatment of CsA or TRPC6 siRNA. Calcineurin activity in the cells was measured using a calcineurin activity assay kit,and TRPC6 expression was examined using Western blotting and RT-PCR. PASMCs proliferation following the treatments was determined using MTT assay. Results：ET-1 activited Calcineurin activity and upregulated TRPC6 expression. TRPC6 expression was decreased in response to the CsA treatment. Calcineurin activity was attenuated after pretreatment with TRPC6 siRNA;however,ET-1-stimulated PASMCs proliferation was inhibited after pretreatment with both CsA and TRPC6 siRNA. Conclusion：Calcineurin and TRPC6 are involved in ET-1-induced proliferation in PASMCs,and the interaction existes between Calcineurin and TRPC6 in PASMCs.

关 键 词：CALCINEURIN NFAT信号通路 TRPC6 ET-1 肺动脉平滑肌细胞 

分 类 号：R543.2[医药卫生—心血管疾病]