FLIP、Smac和survivin蛋白在肺小细胞癌及低分化鳞癌中表达规律及预后相关性研究  

Correlation between expression of FLIP,Smac and survivin protein and prognosis in small cell lung carcinoma and poorly differentiated squamous-cell carcinoma

作　　者：刘换新[1,2] 王炜[3] 张燕[1] 郭琳琅[1] 

机构地区：[1]南方医科大学附属珠江医院病理科,广东广州510282 [2]武警广东总队医院病理科,广东广州510507 [3]广州军区广州总医院病理科,广东广州510010

出　　处：《南京医科大学学报（自然科学版）》2013年第9期1202-1207,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助(81172241)

摘　　要：目的:通过检测小细胞肺癌(small cell lung carcinoma,SCLC)和低分化鳞状细胞癌(poorly differentiated squamous-cell carcinoma,PDSCC)中FLICE抑制蛋白(FLICE inhibitory protein,FLIP)、促凋亡蛋白Smac(second mitochondrial activator of caspase,Smac)、抗凋亡因子survivin的表达,探讨小细胞肺癌及低分化鳞癌中FLIP、Smac和survivin蛋白的表达规律及其与临床病理指标及预后的关系,为临床诊断、治疗及预后判断提供新的理论依据。方法:应用免疫组化S-P法检测肺小细胞肺癌及低分化鳞状细胞癌中FLIP、Smac和survivin阳性表达;应用蛋白印记(Western blot)和逆转录-聚合酶链反应(RT-PCR)检测正常肺组织及两种癌组织中FLIP、Smac、survivin蛋白和mRNA表达水平。结果:73例肺癌患者1年生存率为64.5%。其中小细胞肺癌60.5%,低分化鳞状细胞癌68.6%。FLIP、Smac及survivin在小细胞肺癌及低分化鳞癌中的表达均与年龄、性别、是否吸烟及淋巴结转移无关(P>0.05),但Smac的表达与临床分期及生存率有关(P<0.05);Smac在正常组织表达显著高于癌组织(P<0.05);FLIP及survivin在正常组织中的表达显著低于癌组织(P<0.05)。结论:癌组织中Smac低表达提示低生存率,Smac可作为独立预后指标;FLIP、Smac和survivin联合检测有助于预后判断。Objective：To detect the expressions of FLIP,Smac and survivin in small-cell lung carcinoma （SCLC） and poorly differentiated squamous-cell carcinoma （PDSCC）,and analyze the correlation between the expressions of FLIP,Smac and survivin and clinical pathologic features and prognosis. Methods：The expressions of FLIP,Smac and survivin were detected by S-P immunohistochemical stain in SCLC and PDSCC. Protein and mRNA expressions of FLIP,Smac and survivin in normal lung tissue,SCLC and PDSCC were examined by Western blot and reverse transcription-polymerase chain reaction （RT-PCR）,respectively. Results：One-year survival rate was 64.5%,60.5%,and 68.6%in total population studied,SCLC,and PDSCE,respectively;the expressions of FLIP,Smac and survivin were not related to sex,age,lymph node metastasis and smoking either in SCLC or in PDSCC （P 〉 0.05）. Smac was significantly correlated with clinical stages and survival rates after treatment （P 〈 0.05）. Protein and mRNA expressions of Smac in normal lung tissues were significantly higher than those in tumor tissues （P 〈 0.001）,while protein and mRNA expressions of FLIP and survivin in normal lung tissues were significantly lower than those in tumor tissues （P 〈 0.001）. Conclusion：Low expression of Smac in carcinoma tissues indicates low survival rate,and Smac may be an independent prognostic indicator. The combination of the detection of FLIP,Smac and survivin in cancer tissues may be helpful to predict prognosis.

关 键 词：肺癌 FLIP SMAC SURVIVIN 

分 类 号：R734.2[医药卫生—肿瘤]