基于展示技术的Cd^(2+)结合肽筛选与酵母重金属吸附研究  

Selection of Cd^(2+) Binding Peptides and Yeast Adsorption of Heavy Metals by Display Technology

作　　者：冯侠[1] 江年[1] 陈颖[1] 张薇薇[1] 李学如[1] 茆灿泉[1] 

机构地区：[1]西南交通大学生命科学与工程学院分子进化与应用生物学实验室,四川成都610031

出　　处：《河南农业科学》2013年第9期142-145,共4页Journal of Henan Agricultural Sciences

基　　金：国家"863"计划项目(2006AA06Z353)

摘　　要：利用Cd2+螯合树脂,对噬菌体随机十二肽库进行Cd2+结合肽筛选,并将亲和力较强的结合肽展示在酵母EBY100表面,以期得到耐受和吸附能力具佳的工程菌,为重金属的生物修复提供新的思路。通过筛选共得到6条不同的Cd2+结合肽,其中有2条对Cd2+亲和力较强,将其串联展示于酵母细胞表面。与对照菌相比,酵母工程菌对Cd2+、Ni 2+的吸附率显著提高。酵母工程菌对Cd2+的吸附率随诱导时间的增加而增加,诱导24h时最高,达30.4%,此时酵母工程菌对Ni 2+的吸附率达到32.9%。Cd^2＋ binding peptides were effectire enriched from the commercially phage 12 random peptide library,and six different Cd2＋ binding peptides were obtained,two which had strong affi- nity were selected and connected together in series and displayed on yeast surface by gene recom- bination. The recombinant yeasts showed significant higher adsorption rate for Cd^2＋ and Ni^2＋ as compared with the control. The recombinant yeasts adsorption rate for Cd2＋ increased with the in- crease of the induction time, when the induction time was 24 h, the adsorption rate for Cd2＋ reached the maximum （30.4%） ,and the adsorption rate for Nia＋ reached 32.9%.

关 键 词：重金属Cd2＋结合肽 噬菌体随机肽库 展示技术 酵母 

分 类 号：Q789[生物学—分子生物学]