羧基肽酶E(CPE)在人乳腺腺癌中的表达和功能(英文)  

Expression and function of carboxypeptidase E(CPE) in human breast adenocarcinoma

作　　者：刘换新[1] 周东[2] 游娟[3] 张娟辉[4] 唐松山[3] 唐柏山[5] 

机构地区：[1]武警广东省总队医院病理科,广州510507 [2]南方医科大学附属广东省人民医院神经外科,广州510080 [3]广东药学院基础学院生化教研室,广州510006 [4]武警广东省总队医院妇产科,广州510507 [5]南方医科大学珠江医院血液科,广州510280

出　　处：《复旦学报（医学版）》2013年第5期523-528,533,共7页Fudan University Journal of Medical Sciences

基　　金：教育部留学基金项目(44143012);广东省医学科研基金项目(A2011456);广东药学院基金项目(52104109)~~

摘　　要：目的观察乳腺腺癌中羧基肽酶E(carboxypeptidase E,CPE)介导的神经内分泌肽加工和表达。方法使用RT-PCR、免疫组化、ELISA和Western blot等方法,比较CPE和生长激素释放激素(growth hormone releasing hormone,GHRH)基因和蛋白质水平在42例乳腺癌和21例癌旁正常组织中的表达。结果乳腺癌组织中的CPE基因表达水平为1 024±237,而癌旁正常组织中CPE基因表达水平为805±83(P<0.01)。与乳腺癌旁正常组织比较,癌组织中CPE蛋白质显示了较高的表达(P<0.01)。乳腺癌组织中的GHRH基因表达水平为1 522±457,而癌旁正常组织中为1 067±437,差异有统计学意义(P<0.01)。与乳腺癌旁正常组织比较,癌组织中GHRH蛋白表达被上调(P<0.01)。结论癌组织中CPE和GHRH高表达提示癌细胞中存在较强的由CPE酶介导的神经内分肽转录后加工能力。Objective To observe the expression and processing of carboxypeptidase E （CPE） mediated neuroendocrinopeptide in human breast adenocarcinoma. Methods By using RT-PCR,immunohistochemistry,ELISA,and Western blot techniques,the expression profiles of CPE and growth hormone releasing hormone （GHRH） gene and protein were investigated between 42 human breast tumor tissues and 21 tumoradjacent normal ones. Results The expression level of CPE gene in the breast adenocarcinoma tissues was 1 024＆plusmn;237,while it was 805＆plusmn;83 in the tumoradjacent normal tissues （P〈0.01）.The CPE protein in the breast tumor tissues showed higher expression than in the tumoradjacent normal tissues （P〈0.01）.The expression level of GHRH gene in the breast adenocarcinoma tissues was 1 522＆plusmn;457,while it was 1 067＆plusmn;437 in the tumoradjacent normal tissues （P〈0.01）.The GHRH protein was upregulated in the tumor tissues （P〈0.01）. Conclusions The high expressions of CPE and GHRH suggest that there is a strong CPE mediated neuroendocrinopeptide posttranslational processing in the breast tumor cells.

关 键 词：神经内分泌肽 羧基肽酶E （CPE） 生长激素释放激素（GHRH） 人乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]