rhBMP-7促进大鼠颅骨缝牵张成骨的实验研究  

The rhBMP-7 accelerated the distraction osteogenesis of rat cranial suture

作　　者：张慧[1] 沈庆冉 肖迪[1] 陈建伟[1] 邹淑娟[1] 

机构地区：[1]四川大学华西口腔医院,成都610041 [2]四川省成都市龙泉区第一人民医院口腔科

出　　处：《现代口腔医学杂志》2013年第5期280-282,共3页Journal of Modern Stomatology

基　　金：国家自然科学基金(81070859)

摘　　要：目的观察人重组骨形成蛋白-7(rhBMP-7)对大鼠颅骨矢状缝牵张成骨的影响。方法建立大鼠颅骨矢状缝扩张培养模型,运用组织学及免疫组织化学技术,观察加入rhBMP-7后,受力骨缝中的新骨形成及细胞增殖核抗原(PCNA)的表达,同时检测培养液中ALP的活性变化。结果在加入rhBMP-7的作用下,受力骨缝内成骨反应活跃,PCNA阳性表达细胞明显增多(P<0.05),ALP活性也显著增强(P<0.05)。结论外源性BMP-7有诱导骨缝成骨细胞增殖与分化、从而促进骨缝牵张时新骨的形成。Objective To observe the effect of rhBMP-7 in the distracted cranial sagittal suture of rat. Methods By means of an organ cultured cranial suture model, the expression of proliferating cell nuclear antigen （PCNA）, alkaline pbosphatase （ALP） activity and bone formation within the sutures were examined after treatment of rhBMP-7. Results The results showed that PCNA-positive cells and ALP activity increased significantly after addition of rhBMP-7 （P〈 0.05）, new bone formation within the sutures was enhanced. Conclusion Exogenous BMP - 7 could induce the proliferation and differentiation of osteoblasts in the sutures, which promote new bone formation in the suture of distraction osteogenesis .

关 键 词：骨缝牵张成骨 人重组骨形成蛋白-7 碱性磷酸酶 

分 类 号：R782.2[医药卫生—口腔医学]