胃癌弱差异基因表达谱建立的生物学意义  

Biological significance of profiling of weakly differentially expressed genes in gastric cancer

作　　者：孙伟[1] 高芳[2] 龙启福[1] 王晓龙[3] 朱德锐[1] 顾存林[1] 安娟[1] 党国全[1] 吴穹[1] 

机构地区：[1]青海大学医学院基础医学部,青海省西宁市810016 [2]青海大学附属医院重症ICU科,青海省西宁市810001 [3]青海大学附属医院胃肠肿瘤外科,青海省西宁市810001

出　　处：《世界华人消化杂志》2013年第26期2717-2723,共7页World Chinese Journal of Digestology

基　　金：青海大学医学院中青年教师科研基金资助项目;No.2012KYT-03~~

摘　　要：目的:明确用基因表达水平的贝叶斯分析(Bayesian analysis of gene expression levels,B A G E L)所建立的胃癌弱差异基因表达谱(fold change<1.5)的特异性及生物学意义.方法:Cluster3.0聚类分析基因DNA甲基转移酶3A[DNA(cytosine-5-)-methyltransferase3 a l p h a,D N M T3A]、发育分化增强因子2(development and differentiation-enhancing f a c t o r 2,D D E F2)、分化群59(c l u s t e r o f differentiation 59,CD59)的表达;机器学习和模式识别技术对弱差异基因表达谱(fold change<1.5,P<0.001)进行特征提取;GoMiner软件分析弱差异表达基因D N M T3A、DDEF2、CD59的生物学功能;RT-PCR在实验室验证弱差异表达基因DNMT3A、DDEF2、CD59在胃癌及癌旁配对组织中mRNA的表达水平.结果:从弱差异基因表达谱中得到能够识别样本类别的62个分类特征基因和4个分类能力较强的基因;GoMiner分析表明这组基因参与细胞黏附、细胞吞噬、免疫调节、基因甲基化、转录调控等重要生物学作用;RT-PCR确定基因表达变化与芯片中基因表达谱的数据一致,证实了弱差异基因表达谱的可靠性和灵敏性.结论:通过BAGEL分析得到的fold change<1.5的弱差异基因表达谱灵敏可靠,在肿瘤发生发展中关系密切.AIM： To clarify the specificity and biological significance of profiling of weakly differentially expressed genes （fold change 〈 1.5） in gastric cancer by Bayesian analysis of gene expression levels （BAGEL） analysis. METHODS： The mRNA expression of DNA （cytosine-5-）-methyltransferase 3 alpha （DNMT3A）, development and differentiation-enhancing factor 2 （DDEF2） and cluster of differentiation 59 （CD59） was clustered by Cluster 3.0. Machine study and pattern recognition analysis was used to study the feature of weakly differentially expressed genes. The functions of CD59, DDEF2 and DNMT3A were analyzed using GoMiner. The mRNA expression of CD59, DDEF2 and DNMT3A in tissues was detected by RT-PCR. RESULTS： We have collected 62 classification characteristic genes from 64 weakly differentially expressed genes and obtained 4 strongest classification genes by machine study and pattern recognition analysis. GoMiner analysis showed that these weakly differentially expressed genes were associated with cell adhesion, phagocytosis, immunological regulation, gene methylation and transcription regulation. Furthermore, the differential expression of these genes in tissue samples was confirmed by RT-PCR. CONCLUSION： Profiling of weakly differentially expressed genes by BAGEL analysis is specific and allows to identify genes that are closely related to tumorigenesis.

关 键 词：胃癌 基因表达谱 CD59 DDEF2 DNMT3A 

分 类 号：R735.2[医药卫生—肿瘤]