MADD抑制人前列腺癌PC-3细胞凋亡作用的研究  

Role of MAPK-activating death domain-containing protein in inhibiting apoptosis of human prostate cancer PC-3 cells

作　　者：崔崎[1] 韩玲[1] 

机构地区：[1]新疆医科大学附属中医院泌尿外科,乌鲁木齐830000

出　　处：《重庆医科大学学报》2013年第9期989-993,共5页Journal of Chongqing Medical University

摘　　要：目的:检测含促分裂素原活化蛋白激酶激活死亡结构域蛋白(MAPK-activating death domain-containing protein,MADD)在前列腺癌组织及良性前列腺增生(benign prostatic hyperplasia,BPH)组织中的表达并探讨其对前列腺癌细胞凋亡的作用。方法:收集40例人前列腺癌及35例BPH组织,免疫组化染色技术检测MADD的表达情况;Western blot技术检测MADD在人前列腺癌细胞株(LNCaP、PC-3和PC-3M)和良性前列腺细胞株(BPH1)中的表达情况;应用MADD siRNA转染人前列腺癌PC-3细胞,Western blot检测其MADD、caspase-3和PARP表达,MTT和流式细胞仪检测细胞增殖和凋亡变化。结果:MADD在前列腺癌组织中的表达高于BPH组织(P=0.000);人前列腺癌细胞株LNCaP、PC-3M和PC-3中MADD表达水平明显高于良性前列腺细胞株BPH1(P=0.009、P=0.001、P=0.000),沉默PC-3细胞中MADD表达,导致活化型caspase-3和PARP降解产物表达增加,并诱导PC-3细胞凋亡增加和增殖活力降低(P=0.000)。结论:前列腺癌组织中MADD表达明显增高,MADD在前列腺癌中可能作为凋亡抑制因子发挥功能。Objective：To investigate the expression of MAPK-activating death domain-containing protein （MADD） in prostate caner and benign prostatic hyperplasia（BPH） tissues and its effects on apoptosis of prostate cancer cells. Methods：Totally 40 samples of surgically resected prostate cancer tissues and 35 samples of BPH tissues were collected and MADD expressions in the samples were detected using immunohistochemistry. MADD protein expressions in benign prostatic cell line BPH1 and prostate cancer cell lines （LNCaP,PC-3and PC-3M） were detected by Western blot. MADD siRNA was transfected into PC-3 cells,MADD, caspase-3 and PARP expression, cell viability and apoptosis were analyzed by Western blot, MTF and flow cytometry. Results：Expressions of MADD in prostate cancer tissues were significantly upregulated compared with those of the BPH tissues（P=0.000）. Expressions of MADD in prostate cancer cell lines LNCaP,PC-3M and PC-3 were obviously higher than those in benign prostatic cell line BPH1 （P=-0.009,P= 0.001 ,P=0.000, respectively）. Knockdown of MADD in PC-3 cells enhanced expression of cleaved caspase-3 and cleaved PARP, as well as decreased cell viability and increased cell apoptosis（P=0.000, respectively）. Conclusions：Expression of MADD increases obviouslv in nrostate cancers. MADD mav act as an inhihitnr of anontosis as well as a therapeutic target of prostate cancer.

关 键 词：含促分裂素原活化蛋白激酶激活死亡结构域蛋白 前列腺癌 良性前列腺增生 凋亡 增殖 

分 类 号：R737.25[医药卫生—肿瘤]