甲基营养菌MP688胞外多糖合成的影响因素研究  

Research on Factors Influencing Extracellular Polysaccharides Syn thesis in Methylovorus sp. MP688

作　　者：葛欣[1] 韩月梅[1,2] 熊向华[1] 汪建华[1] 刘党生[2] 张惟材[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071 [2]沈阳药科大学,辽宁沈阳110016

出　　处：《生物技术通讯》2013年第5期655-658,共4页Letters in Biotechnology

基　　金：国家自然科学基金(31100024);国家科技支撑计划(2007BAI46B01)

摘　　要：目的：考察培养基组分和发酵条件对甲基营养菌MP688合成胞外多糖的影响，确定最主要的影响因素。方法：将甲基营养菌MP688接种到基础培养基中，通过改变基础培养基的氮源、培养温度、初始pH值和培养时摇床转速等条件，检测在每种条件下培养5 d后发酵液中的多糖含量，确定每种因素的最适范围；进而选取8个因素，通过Plackett-Burman实验设计12组实验，通过检测每种组合条件下的多糖产量和结果统计分析，确定影响多糖合成的最主要因素。结果：甲基营养菌合成胞外多糖的最适氮源为硝酸钠，最适温度为30-37°C，最适pH值为6.5-7.0，最适摇床转速为200-250 r/min；甲醇、硝酸钠、初始pH值和接种量是MP688合成多糖的主要影响因子。结论：运用Plack-ett-Burman实验设计筛选到甲基营养菌MP688胞外多糖合成的主要影响因子，MP688是具有多糖生产潜力的菌株。Objective： To investigate the main factors influencing extracellular polysaccharides synthesis in Methy-lovorus sp. MP688. Methods： Methylovorus sp. MP688 was inoculated into culture media containing various nitro-gen sources and different initial pH, and they were cultured at different temperature or on rotator at different speed. Extracellular polysaccharides concentration was determined after 5 days＇ cultivation. The Plackett-Burman de-sign was applied to screen the significant factors that influence the yield. Results： The optimum nitrogen source for producing extracellular polysaccharides was sodium nitrate. The optimum temperature was 30-37℃. The opti-mum initial pH was 6.5-7.0. The optimum rotator speed was 200-250 r/min. As well, methanol, sodium nitrate, initial pH and inoculum size were identified to be the most significant factors. Conclusion： Significant factors were screened out by Plackett-Burman design and statistical analysis. Methylovorus sp. MP688 was proved to be a promising candidate for producing extracellular polysaccharides.

关 键 词：甲基营养菌 胞外多糖 Plackett—Burman实验设计 

分 类 号：Q935[生物学—微生物学]