^(99)Tc^m-黄体生成素释放激素在荷人乳腺癌裸鼠体内的生物学分布  

Biological distribution of ^(99)Tc^m-luteinzing hormone-releasing hormone in nude mice bearing human breast cancer

作　　者：年娣[1] 孙俊杰[1] 时鹏[2] 

机构地区：[1]蚌埠医学院核医学教研室,安徽蚌埠233030 [2]蚌埠医学院第一附属医院神经内科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2013年第10期1238-1240,共3页Journal of Bengbu Medical College

基　　金：蚌埠医学院科研资助项目(BY1013)

摘　　要：目的:研究99Tcm-黄体生成素释放激素(99Tcm-LHRH)在荷人乳腺癌裸鼠体内的生物学分布,探讨LHRH用于肿瘤诊断和靶向治疗的价值。方法:直接标记法99Tcm标记LHRH;25只荷瘤鼠随机分成5组,每组5只;荷人乳腺癌SK-BR3裸鼠尾静脉注射99Tcm-LHRH后断头处死小鼠,测定99Tcm-LHRH在各组荷瘤鼠体内的放射性分布,计算每克组织放射性占总注入放射性的百分比。结果:99Tcm-LHRH放化纯度>95%,标志物稳定性好。荷瘤鼠注入99Tcm-LHRH后4 h,肿瘤/血液及肿瘤/肌肉的比值分别为12.89±1.67、36.81±5.64,99Tcm-LHRH主要分布于肿瘤、肝脏、血液,脑、肌肉等组织中放射性分布极低。结论:99Tcm-LHRH高特异地与人乳腺癌细胞结合,在乳腺癌显像和靶向治疗中有潜在的应用前景。Objective： To study the biodistribution of 99Tcm-luteinzing hormone-releasing hormone （99Tcm-LHRH） in nude mice bearing human breast cancer,and to evaluate the effect of cancer diagnosis and targeted radiotherapy with 99Tcm-LHRH in nude mice bearing human breast cancer. Methods：LHRH was directly radio labeled with 99Tcm. Twenty-five nude mice were randomly divided into five groups, five in each. Nude mice bearing SK-BR3 breast cancer cells were injected with 99Tcm-LHRH by vein caudalis, and sacrificed. Detect the biodistribution of 99Tcm-LHRH and calculated per gram Organization percentage injected dose rate. Results：The result showed that radiochemical purity of 99Tcm-LHRH was more than 95%. The ratios of tumor to blood and tumor to muscle were 12.89 ± 1.67, and 36.81 ± 5.64, respectively. The biodistribution experiment showed that 99 Tcm-LHRH uptake by liver, blood, tumor site was higher than by muscle and brain. Conclusions：99 Tcm-LHRH has good specificity to human breast cancer cell. It may be used in the targeted diagnosis and therapy of breast cancer.

关 键 词：乳腺肿瘤 黄体生成素释放激素 荷瘤裸鼠 生物学分布 

分 类 号：R737.9[医药卫生—肿瘤]