异源表达AtCIPK23基因对马铃薯试管薯结薯及耐低钾抗性的影响  

Effects of AtCIPK23 Gene Ectopic Expression on vitro Tuberization and Tolerance against Low-K^+Stress in Potato

作　　者：张烨[1] 邹雪[1] 吴明阳[1] 王西瑶[1] 

机构地区：[1]四川农业大学农学院,四川温江611130

出　　处：《四川农业大学学报》2013年第3期249-253,共5页Journal of Sichuan Agricultural University

基　　金：国家现代产业技术体系四川马铃薯创新团队项目(川农业[2008]225号)

摘　　要：【目的】研究低钾处理下异源表达AtCIPK23基因对马铃薯试管薯结薯的影响。【方法】以马铃薯品种鄂薯3号(W)及其转AtCIPK23基因的株系L1、L23和L34为材料,通过试管薯诱导体系,研究马铃薯试管薯在不同钾浓度处理下的单瓶结薯数、薯块直径以及植株和试管薯的钾含量,试管薯淀粉与蔗糖的含量。【结果】2mmol/L和10mmol/L处理下,L1、L23和L34的单瓶结薯数、薯块直径均高于对照W,且植株和试管薯的钾含量,试管薯淀粉与蔗糖的含量均高于对照W。正常钾(20mmol/L)处理下,对照与转基因各株系间各指标的差异不显著。【结论】异源表达AtCIPK23基因提高了马铃薯试管薯对低钾的抗性。[Objective] The purpose of this study was to research effects of ectopic expression AtCIPK23 gene on potato tuberization in vitro under low potassium treatments. [Method] Three transgenic potato lines （L1, L23 and L34 ） and non-transgenic control W （E3 cultivar） were used to explore tuber growth in low K＋ condition by microtuber induction system. The number of tu- bers in single bottle, diameter of potato, potassium content in potato plant and microtuber, the content of microtuber starch and sucrose were determined under different concentrations of potas- sium. [Results] The number and diameter of mirotuber in transgenic lines L1, L23 and L34 were higher than that in W under 2 mmol/L and 10 mmol/L potassium concentration treatments. Po tassium content in plant and microtuber, sucrose and the starch content in microtuber of transgen ic lines were higher than that of non-transgenic lines in these potassium concentrations. In 20 mmol/L potassium concentration, these indexes between transgenic lines and control were no-sig nificant different. [Conclusion] AtCIPK23 gene ectopic expression in potato could increase the resistance to low potassium condition.

关 键 词：马铃薯 AtCIPK23 耐低钾 试管薯 

分 类 号：Q789[生物学—分子生物学]