利用糖厂滤泥生产根瘤菌菌剂  

Production of rhizobia inoculant using sugar mill filter mud

作　　者：刘晓举[1] 陈宝娣[1] 郭继强[1] 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2013年第3期187-190,共4页Journal of Dalian Polytechnic University

基　　金：国家科技支撑计划项目(2011BAE16B02)

摘　　要：测定了甘蔗糖厂滤泥的成分,确定滤泥中含有足够根瘤菌生长所需要的营养元素。在滤泥中接种根瘤菌进行培养实验,分析了根瘤菌在滤泥中的生长状况。利用单因素实验和响应面的方法,分析优化了大豆根瘤菌在滤泥中生长的最佳条件:温度29℃,pH 7.5,总糖总氮比7∶10,湿度72.97%,接种量51.3mL/kg。脱水滤泥可以作为根瘤菌生长的载体。The composition of the cane sugar mill filter mud suggested that there are enough nutritive element for the growth of rhizobium in the filter mud. The cell growth of rhizobia in the filter mud was analyzed. With single factor test and response surface methodology, the optimum condition for growth of rhizobia is： temperature 29 ~C, pH 7.5, the ratio of total sugar and total nitrogen 7 ： 10, humidity 72.97%, inoculum size of filter mud 51.3 mL/g. This suggested that the dehydration filter mud can be used to the growth of rhizobia.

关 键 词：滤泥 大豆根瘤菌 根瘤菌菌剂 

分 类 号：TS249.4[轻工技术与工程—制糖工程]