ELL慢病毒表达载体的构建及其感染前列腺癌PC3细胞的研究  

Construction of ELL gene lentiviral expression vector and its infection in human prostate cancer PC3 cells

作　　者：刘凌琪[1] 吴天鹏[1] 杨嗣星[1] 何志嵩[2] 辛殿祺[2] 郭应禄[2] 艾军魁[3] 王洲[3] 

机构地区：[1]武汉大学人民医院泌尿外科,430060 [2]北京大学泌尿外科研究所 [3]美国匹兹堡大学泌尿外科

出　　处：《现代泌尿生殖肿瘤杂志》2013年第4期225-229,共5页Journal of Contemporary Urologic and Reproductive Oncology

基　　金：国家自然科学基金项目青年项目(81101948)

摘　　要：目的构建ELL基因的慢病毒表达质粒,探讨其感染人前列腺癌PC3细胞的可行性。方法将含有全长ELL的质粒和慢病毒表达载体经双酶切后连接,构建成重组慢病毒载体质粒pCDH1-MCS1-EF1-copGFP-ELL。对慢病毒载体质粒进行双酶切和测序鉴定后,制备包装病毒并转染人前列腺癌细胞系PC3。结果慢病毒载体质粒pCDH1-MCS1-EF1-copGFP-ELL的酶切和测序结果与预计的序列一致。慢病毒感染人前列腺癌PC3细胞后能稳定高表达ELL。结论成功构建了ELL的慢病毒表达载体,ELL可被成功转染入人前列腺癌细胞。Objective To construct ELL gene lentiviral feasibility of its infection in human prostate cancer PC3 cells expression plasmid and explore the Methods Containing the full-length ELL plasmid and lentivirus vector were double restriction digested and connected to construct recom- binant lentiviral expression vector pCDH1-MCS1-EFI-copGFP- ELI.. Identified by double restriction enzyme digestion and sequencing analysis, recombinant plasmid was packaged and used to infect hu- man prostate cancer PC3 cells. Results Double restriction enzyme digestion and sequencing analy- sis of lentiviral vector plasmid pCDH1-MCSI-EFI-copGFP- ELL showed the expected results. Hu- man prostate cancer PC3 cells infected by lentiviral vector can overexpress ELL protein. Conclu- sions ELL lentiviral expression vector was successfully constructed. ELL can be successfully trans- fected into human prostate cancer cells.

关 键 词：ELL 慢病毒表达载体 

分 类 号：R737.25[医药卫生—肿瘤]