顽拗性黄皮胚脱水过程中核酸、蛋白质代谢的变化  

Metabolism of Nucleic Acids and Proteins in Recalcitrant Wampee (Clausena lansium)Embryos during Desiccation

作　　者：王艳[1] 李韶山[2] 何军贤[3] 傅家瑞[4] 

机构地区：[1]暨南大学生命科学和技术学院,中国广东广州510632 [2]华南师范大学激光生命科学研究所,中国广东广州510631 [3]四川大学生物系,中国四川成都610064 [4]中山大学生命科学学院,中国广东广州510275

出　　处：《激光生物学报》2000年第3期165-169,共5页Acta Laser Biology Sinica

基　　金：国家自然科学基金资助项目!( 3 94 70 0 82 );广东省自然科学基金资助项目!( 94 0 61 8)

摘　　要：黄皮种子发育晚期 ,胚内核酸、蛋白质合成能力增强 ,而花生胚的核酸、蛋白质合成能力在发育晚期则呈下降趋势。黄皮胚的发育在达到生理成熟后维持着活跃的生理代谢并转入萌发状态 ;而花生胚的代谢活性逐步降低并转入生理静止状态。脱水处理引起生理成熟期黄皮胚核酸、蛋白质合成能力急剧下降 ,核酸水解酶活性增强。不同程度脱水的黄皮胚吸胀 2 4 h,核酸、蛋白质的合成能力随脱水程度的加深而降低 ;生物大分子代谢能力的变化是顽拗性黄皮种子脱水敏感性的分子基础。Synthetic rate of nucleic acids and proteins in embryos of recalcitrant wampee seeds were increased gradually in the late developmental stage(7190DAF).After physiological maturation, wampee seeds maintained active metabolism and transferred into germinating state. However, in the orthodox peanut seeds, synthetic rate of nucleic acids and proteins were decreased with the maturation of seeds (6585DAP).Peanut seeds maintained lower level of metabolism, and entered stationary state when maturation. During the processes of water loss in wampee embryos, the ability of macromolecule's synthesis was rapidly decreased and hydrolysis of nucleic acids was obvioulsy enhanced. Imbibing for 24h ,synthetic rates of nucleic acids and proteins in dehydrated embryos of wampee were decreased with water loss. Metabolism of biomacromolecules in dehydrated embryos of peanut could be completely recovered by rehydration. It was suggested that desiccation sensitivity in recalcitrant wampee seeds was related to the metabolism of nucleic acids and proteins.

关 键 词：顽拗性种子 脱水敏感性 核酸 蛋白质代谢 黄皮 

分 类 号：S330.2[农业科学—作物遗传育种]