淋巴细胞溶质蛋白1调控弥漫性大B细胞淋巴瘤细胞的增殖初探  

Lymphocyte cytosolic protein 1 regulates proliferation of diffuse large B-cell lymphoma cells

作　　者：张竹红[1,2] 田亚平[1] 

机构地区：[1]解放军总医院生化科,北京100853 [2]南开大学医学院,天津300071

出　　处：《解放军医学院学报》2013年第10期1052-1054,共3页Academic Journal of Chinese PLA Medical School

基　　金：国家科技支撑计划项目(2009BAI86B05)~~

摘　　要：目的探索弥漫性大B细胞淋巴瘤中淋巴细胞溶质蛋白1(lymphocyte cytosolic protein 1,LCP1)表达的生物学意义及对细胞增殖的影响。方法用Wstern Blot的方法观察LCP1在正常人B淋巴母细胞系和多种弥漫性大B细胞淋巴瘤细胞系中的表达情况;进而在高表达LCP1的弥漫性大B细胞淋巴瘤(diffuse large B-cell lymphom,DLBCL)细胞系OCI-LY19中将其沉默,使用CCK8试剂盒检测其对细胞活力的影响;并用PI染色的方法检测LCP1对OCI-LY19细胞的细胞周期的影响。结果 LCP1在DLBCL细胞中的表达量明显高于正常人B淋巴母细胞系,沉默LCP1后发现OCI-LY19细胞活力下降,且细胞周期进展明显被抑制。结论 LCP1与DLBCL细胞增殖密切相关,提示其是一个重要DLBCL生物标记。Objective To study the biological significance of lymphocyte cytosolic protein 1 （LCP1） expression in diffuse large B-cell lymphoma （DLBCL） cells and its effect on proliferation of DLBCL cells. Methods Expression of LCP1 in healthy human B lymphoblastoid cells and various DLBCL cells was detected by Western blot. Over-expressed LCP1 was silenced in DLBCL cell line OCI-LY19. Vitality of the cells was tested with a CCK8 kit. Effect of LCP1 on cell cycle of OCI-LY19 cells was observed with PI staining. Results The expression level of LCP1 was significantly higher in DLBCL cells than in healthy human B lymphoblastoid cells. The vitality of OCI-LY19 cells decreased and the progressive cell sycle of OCI-LY19 cells was inhibited after LCP1 was silenced. Conclusion LCP1 is closely related with the proliferation of DLBCL cells and is an important biological marker for DLBCL.

关 键 词：微小RNA 淋巴瘤 细胞增殖 

分 类 号：R73-3[医药卫生—肿瘤]