骨髓基质细胞移植对大鼠脊髓损伤后环氧化酶-2和血管内皮生长因子表达的影响  

Effect of bone marrow stromal cells transplantation on expressions of cyclooxygenase-2 and vascular endothelial growth factor in rats following spinal cord injury

作　　者：于德水[1] 曹阳[1] 梅晰凡[1] 王岩松[1] 闫鹏[1] 吕刚[1] 

机构地区：[1]辽宁医学院附属第一医院骨科,辽宁锦州121001

出　　处：《解放军医学院学报》2013年第10期1059-1062,共4页Academic Journal of Chinese PLA Medical School

基　　金：辽宁省博士科研启动基金项目(20121096);辽宁医学院博士及出国进修一年以上教师科研启动基金项目(Y2012B011)~~

摘　　要：目的观察骨髓基质细胞(bone marrow stromal cells,BMSCs)移植对大鼠脊髓损伤后环氧化酶-2(cyclooxygenase-2,COX-2)和血管内皮生长因子(vascular endothelial growth factor,VEGF)蛋白表达的影响。方法大鼠随机分为3组:A为假手术组,B为细胞培养基(DMEM)对照组,C为BMSCs移植组。B组和C组大鼠进行脊髓压迫损伤模型制作。脊髓损伤后,B组和C组大鼠分别给予DMEM培养液或BMSCs脊髓损伤周围区注射。术后应用Western Blot方法检测COX-2、VEGF蛋白在损伤脊髓组织中的表达变化。结果 COX-2、VEGF蛋白在A组大鼠未损伤脊髓组织中均低水平表达。B组与A组相比,脊髓损伤后COX-2蛋白表达水平明显增加(P<0.01),VEGF蛋白表达水平则短暂显著增加(P<0.05)。C组与B组相比,COX-2和VEGF蛋白表达水平均显著增加。结论 BMSCs移植能够增加脊髓损伤后COX-2、VEGF蛋白的表达。Objective To observe the effect of bone marrow stromal cells （BMSCs） transplantation on expression of cyclooxygenase-2 （COX-2） and vascular endothelial growth factor （VEGF） protein in rats following spinal cord injury （SCI）. Methods Adult rats were randomly divided into group A （sham operation group）, group B （DMEM control group）, and group C （BMSCs transplantation group）. A rat spinal cord compression injury model was established. Rats in groups B and C were injected with BMSCs or DMEM after SCI. Expression of COX-2 and VEGF protein in rats following SCI was detected by Western blot. Results The expression level of COX-2 and VEGF protein was low in group A. The expression level of COX-2 and VEGF protein was significantly higher in group B than in group A and in group C than in group B （P〈0.01, P〈0.05）. Conclusion BMSCs transplantation can up-regulate the expression of COX-2 and VEGF protein in rats following SCI.

关 键 词：脊髓损伤 骨髓基质细胞 环氧化酶-2 血管内皮生长因子 细胞移植 

分 类 号：R-332[医药卫生]