Effects of Membrane Lipids on the Electron Transfer Activity of Cytochrome b_6f Complex from Spinach  

膜脂对菠菜细胞色素b_(6)f复合体电子传递活性的影响(英文)

作　　者：阎久胜[1] 毛大璋[1] 陈晖 匡廷云[1] 李良璧 

机构地区：[1]中国科学院植物研究所光合作用基础研究开放实验室,北京100093

出　　处：《Acta Botanica Sinica》2000年第12期1267-1270,共4页Acta Botanica Sinica（植物学报：英文版）

基　　金：The State Key Basic Research and Development Plan(G1998010100);Innovative Foundation of Laboratory of Photosynthesis Basic Research Insitute of Botany,The Chinese Academy of Sciences

摘　　要：A lipid_depleted cytochrome b 6f (Cyt b 6f) preparation was obtained from spinach (Spinacia oleracea L.) chloroplasts. Upon reconstitution of this preparation with the membrane lipids purified from spinach thylakoid, the effects of different membrane lipids on the electron transfer activity were studied. The results show that the electron transfer activity of Cyt b 6f is obviously stimulated to different extents, respectively, by monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), phosphatidylcholine (PC), phosphatidylglycerol (PG) and sulfoquinovosyldiacylglycerol (SQDG), and that the extents of stimulation may be closely related to the charge of the membrane lipids. The stimulation of non_charged lipids (MGDG, DGDG) and neutrally_charged lipid (PC) was high with a maximum enhancement of 89%, 75% and 77%, respectively; but the stimulation of two kinds of negatively_charged lipid (PG and SQDG) was relatively low with a maximum enhancement of 43% and 26%, respectively.利用从菠菜 (SpinaciaoleraceaL .)叶绿体分离、纯化出的缺失膜脂的细胞色素b6f蛋白复合体 (Cytb6f)制剂与从菠菜类囊体分离、纯化的膜脂进行体外重组 ,检测了不同膜脂对Cytb6f催化电子传递活性的影响。结果表明 :被检测的 5种膜脂 ,即单半乳糖基甘油二酯 (MGDG)、双半乳糖基甘油二酯 (DGDG)、磷脂酰胆碱 (PC)、磷脂酰甘油(PG)和硫代异鼠李糖基甘油二酯 (SQDG)对Cytb6f催化电子传递的活性均有明显的促进作用 ,但促进的程度各不相同 ,这可能与这些膜脂分子的带电性质密切相关。不带电荷的MGDG和DGDG及分子整体呈电中性的PC对促进Cytb6f催化电子传递的活性非常有效 ,可分别使其活性提高 89%、75 %和 77% ;而带负电荷的PG和SQDG对活性的促进作用则相对较弱 ,仅可使其活性分别提高 43%和 2 6 %。

关 键 词：cytochrome b_(6)f complex thylakoid membrane lipid RECONSTITUTION electron transfer 

分 类 号：Q945.11[生物学—植物学]