染色体消失法在小麦育种中应用的研究  

A Study on Application of“Chromosome Elimination” in Wheat Breeding

作　　者：李大玮[1] 欧阳平[1] 邱纪文[1] 戴威廉 

机构地区：[1]中国科学院遗传研究所植物细胞与染色体工程国家重点实验室,北京100101 [2]美国马里兰大学全球华人事物研究中心

出　　处：《农业生物技术学报》2000年第1期17-21,T002,共6页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金!39650001;中科院重大科研项目!KZ951-A_1-101;中科院特别支持项目!KZ95T-02

摘　　要：用不同世代的小麦杂种为母本和鸭茅状摩擦禾进行属间杂交。利用杂合子发育过程中来自父本的染色体完全、迅速地消失得到大量单倍体胚。借助于胚培养和染色体加倍技术从杂合体小麦快速得到性状稳定的纯系。本文首次继花药培养法之后向读者介绍另一种以常规育种与生物技术手段相结合的小麦单倍-二倍化育种方法。Using wheat hybrids as female parents and Tripsacum dactyloides as pollen parent intergeneric crosses were made. Haploid embryos which only with chromosome of female parent were obtained due to the chromosome elimination of pollen parent quickly and completely during the heterozygote development. The stable pure lines (DH lines) from wheat hybrids were obtained with the aid of embryo culture and chromosome doubling. Following anther culture method a new method of wheat haploid breeding which is established on combination of conventional breeding with biotechniques is first introduced into readers in this paper.

关 键 词：小麦 鸭茅状摩擦禾 染色体消失 单倍体育种法 

分 类 号：S512.103[农业科学—作物学]