一种检测一次性使用卫生用品致病菌的多重PCR方法  

Establishment of multiplex PCR detective method for three pathogenic bacteria in disposable hygiene products

作　　者：赵琢[1] 栗建永[1] 李晶[1] 贾晓川[1] 张园[1] 赵黎华[1] 

机构地区：[1]天津出入境检验检疫局工业产品安全技术中心,天津300191

出　　处：《工业微生物》2013年第5期68-73,共6页Industrial Microbiology

基　　金：国家质检总局科技计划项目(2012IK122)

摘　　要：建立了一种多重PCR方法,可以快速检测一次性使用卫生用品中的绿脓杆菌、金黄色葡萄球菌及大肠杆菌。通过单重及多重PCR对3种致病茵的目的基因进行特异性扩增,其中单重检出限可分别达到2.0609 pg、0.8127 pg和0.6671 pg。该方法具有良好的检测特异性,实现了一次性使用卫生用品中致病茵的快速检测。A multiple PCR method was established for detecting Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli in disposable hygiene products. 3 kinds of pathogenic bacteria gene were amplified by single and multiple PCR. The single detection limits could reached 2. 060 9 pg, 0. 812 7 pg and 0. 667 1 pg, respectively. The results indica- ted that this detective method was very specific and could rapidly detect 3 kinds of pathogenic bacteria in disposable hygiene products.

关 键 词：PCR 一次性使用卫生用品 绿脓杆菌 金黄色葡萄球菌 大肠杆菌 

分 类 号：R155[医药卫生—营养与食品卫生学]