重复经颅磁刺激对抑郁模型大鼠行为的改善作用及机制  

The effect of repetitive transcranial magnetic stimulation on the changes of depressive like behaviors in chronic unpredictable mild stress model rats and its possible mechanism

作　　者：刘高华[1] 陈冠婕[1] 陈怡环[1] 杨帆[1] 张瑞国[1] 彭正午[1] 谭庆荣[1] 

机构地区：[1]第四军医大学西京医院心身科,西安710032

出　　处：《中华行为医学与脑科学杂志》2013年第9期769-772,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（81171285）;陕西省自然科学基金项目（2013JQ4041）

摘　　要：目的观察重复经颅磁刺激（repetitivetranscranialmagneticstimulation，rTMS）对慢性不可预见性温和应激（chronicunpredictablemildstress，CUMS）抑郁模型大鼠行为的改善作用及对脑源性神经营养因子（brainderivedbeurotrophicfactor，BDNF），白介素-1β（IL-1β），核因子KB（nuclearfactor—KB，NF—KB）表达的影响。方法32只成年雄性SD大鼠随机分为Control组、Control＋rTMS组、CUMS组、CUMS＋rTMS组。CUMS＋rTMS组接受为期21d的造模处理，随后接受连续7d的rTMS干预；CUMS组大鼠也进行21d的造模处理，但是接受连续7d的rTMS假刺激；Control和Control＋rTMS组正常饲养21d后，接受相应的rTMS假刺激或真刺激，为期7d。各组在处理结束后采用糖水偏好实验、强迫游泳实验、旷场实验检测大鼠抑郁样行为表现，并且在行为学实验完成后处死大鼠，以蛋白质印迹法（Westernblot）检测海马BDNF、NF．KB的表达水平，以酶联免疫吸附法（ELISA）检测海马IL-1β的含量。结果1．rTMS对CUMS大鼠抑郁样行为的影响：糖水偏好实验中，CUMS组大鼠的糖水偏好系数（0．67±0．06）较Control组（0．91±0．04）明显减小，而CUMS＋rTMS组大鼠的糖水偏好系数（0．83±0．08）增加；强迫游泳实验中，CUMS组大鼠的不动时间[（26．88±11．33）S]较Control组[（15．22±6．75）S]延长，而CUMS＋rTMS组大鼠不动时间[（18．41±6．95）s]缩短；旷场实验中，CUMS组大鼠的自发活动总距离[（849．17±769．01）cm]与进入旷场中心区次数[（7．42±5．68）次]均显著低于Control组[（6224．81±1403．20）cm，（22．86±3．72）次]，而CUMS＋rTMS组大鼠的自发活动总距离[（4105．574-1516．92）cm]与进入旷场中心区次数[（21．25±3．45）次]增多，差异均有统计学意义（均P〈0．05）。Control组与Control＋rTMS组的上述行为学指标之间均差异无统计学意义。2．rTMS对CUMS大鼠海马BDNF、NF—KObjective To investigate the effect of repetitive transeranial magnetic stimulation （rTMS） on the depressive like behaviors and expression of brain derived neurotrophic factor （BDNF）, IL-1β and NF-KB of hippocampal in chronic unpredictable mild stress （CUMS） rats. Methods Thirty-two adult male rats were ran- domly divided into four groups （ n = 8 ） ： Control group, Control ＋ rTMS group, CUMS group and CUMS ＋ rTMS group. The sucrose preference test, forced swim test and open field test were used to evaluate depressive like be- haviors for each groups. In addition, the expression of BDNF, NF-KB and IL-1 β in hippocampal were detected by westem blot and ELISA after behavioral test, respectively. Results 1. The effects of rTMS on depressive like be- haviors of CUMS rats ： in the sucrose preference test, the sucrose preference rate of CUMS rats （0.67 ±0.06） was significantly lower than Control group （0.91± 0. 04 ） , which was higher in the CUMS ＋ rTMS group （0.83 ±0.08 ）. In the forced swim test, the immobility time of CUMS group （ （26.88 ±11.33 ） s） was longer than Control group （ （ 15.22 ±6.75 ） s） and CUMS ＋ rTMS group （ （ 18.41 ±6.95 ） s）. In the open field test, both the total dis- tance travelled and number of central area entry times of CUMS group（ （ 849. 165 ±769.01 ） cm, （7.42 ±5.68 ） ） were significantly shorter （ （6224.81 ±1403.2）cm） and smaller （22.86 ±3.72 ） than Control group, and those of the CUMS ＋ rTMS were longer （ （4105.57 ±1516.92） cm） and larger （21.25 ±3.45 ）. All the behavioral results were statistically significant （P 〈 0.05 ）. And of all the aforementioned behavioral parameters, there were no signifi- cant differences between Control group and Control ＋ rTMS group（P〉0.05 ）. 2. The effects of rTMS on the hipp- ocampal expressions of BDNF, NF-KB and IL-1β in CUMS rats：compared with Control group, the hippocampal ex- pression of BDNF in CUMS rats was significantly d

关 键 词：重复经颅磁刺激 脑源性神经营养因子 核因子KB 白介素-1 β 大鼠 

分 类 号：R749.4[医药卫生—神经病学与精神病学]