5-氨基酮戊酸对人舌麟癌Tca8113细胞株体外光动力杀伤作用的基础研究  

Study on killing effect of photodynamic therapy using 5-aminolevulinic acid on Tca8113 cells

作　　者：张瑞[1] 张朋[2] 刘伟伟[2] 

机构地区：[1]郑州市中心医院口腔科,郑州450007 [2]郑州大学口腔医学院修复科,郑州450052

出　　处：《医药论坛杂志》2013年第10期9-10,共2页Journal of Medical Forum

基　　金：郑州市2012年重点科技攻关项目(121PPTGG504-8)

摘　　要：目的初步研究体外5-氨基酮戊酸介导的光动力疗法对人舌鳞癌Tca8113细胞的杀伤效应。方法体外培养Tca8113细胞,以5-氨基酮戊酸为光敏剂,半导体激光治疗仪给予光动力疗法,采用MTT法检测光敏剂不同孵育时间、不同光敏剂浓度对Tca8113细胞抑制率的影响。结果 5-ALA-PDT作用后,Tca8113细胞生长受到抑制,孵育时间和浓度效应关系显著(P<0.05)。药物最佳作用浓度为1mmol/l,最佳孵育时间为6h。结论 5-氨基酮戊酸-光动力疗法能有效杀伤Tca8113细胞,光敏剂孵育时间、光敏剂浓度是影响疗效的重要因素。Objective To investigate the effect of 5 -ALA -mediated PDT on human tongue squamous cell carcinoma cell line Tca8113. Methods The Tca8113 cells were routinely cultured in vitro,added with different concentration of photosensitizer 5 - ALA, then lighted with laser meter and observed the time - concentration effect of the drug by MTT. Results The effect of cytotoxicity of 5 - ALA was found on Tca8113 ceils. The inhibition rate of ceils is significantly different with the 5 - ALA concentration and incubation time （ P 〈 0. 05 ）. The strongest photoinactivation effect occurred in the co - cultured cells with 1 mmol/l 5 - ALA for 6 hours. Conclusion 5 - ALA - PDT has distinctive inhibition effect on Tca8113 cells, which correlated with concentration and incubation time of 5 -ALA.

关 键 词：5-氨基酮戊酸 光动力疗法 TCA8113细胞 

分 类 号：R739.8[医药卫生—肿瘤]