PTEN在乳腺癌对顺铂化疗敏感性中的作用及其机制研究  

Effects and Mechanisms of PTEN on the Cisplatin Sensitivity of Human MCF-7 Breast Cancer Cells

作　　者：吴新刚[1] 向安萍[1] 陈杰[1] 

机构地区：[1]岳阳职业技术学院医学基础部,湖南岳阳414000

出　　处：《肿瘤药学》2013年第5期340-343,352,共5页Anti-Tumor Pharmacy

基　　金：湖南省教育厅基金资助项目(11C1284)

摘　　要：目的探讨PTEN在乳腺癌对顺铂化疗敏感性中的作用及其可能的机制。方法应用脂质体转染技术建立PTEN沉默的乳腺癌细胞系MCF-7/PTENi,通过MTT实验检测其对顺铂的敏感性,并通过Western印迹法检测细胞中Bcl-2的表达水平;应用小分子抑制剂ABT-737抑制Bcl-2表达后,再次检测MCF-7/PTENi对顺铂的敏感性。结果 MCF-7/PTENi和阴性对照细胞(MCF-7/NC)对顺铂的IC50值分别为(16.01±3.03)和(7.86±0.85)μM,两者差异有统计学意义(P=0.003)。MCF-7/PTENi细胞中Bcl-2的表达水平明显高于MCF-7/NC细胞(P=0.015)。2μM ABT-737处理的MCF-7/PTENi和MCF-7/NC细胞对顺铂的IC50值比较无显著性差异(P=0.087)。结论 PTEN沉默可降低乳腺癌细胞对顺铂的敏感性,其机制可能与其增加Bcl-2的表达有关。Objective To study the roles of PTEN in the cisplatin chemosensitivity of breast cancer and its possible mecha-nisms. Methods PTEN-silenced MCF-7 cell line （MCF-7/PTENi） was established by lipofectamine transfection. The sensitiv-ity of MCF-7/PTENi cells to cisplatin was tested by MTT treated with or without ABT-737, a novel small molecule inhibitor of Bcl-2 protein. The expression of Bcl-2 was detected by Western blotting. Results The IC50 of MCF-7/PTENi cells treated by cisplatin was 16.01±3.03μM, and it was significantly higher than that of MCF-7/NC cells which was 7.86±0.85μM, with statistical difference （P=0.003）. The expression of Bcl-2 in MCF-7/PTENi cells was significantly higher than that of MCF-7/NC cells （P=0.015）. No significant difference was found between the MCF-7/PTENi and MCF-7/NC cells treated with 2μM ABT-737 and cisplatin （P=0.087）. Conclusion Loss of PTEN expression decreased the chemosensitivity of breast cancer cells to cisplatin, and its mechanism may be involved with the increase of Bcl-2 expression.

关 键 词：PTEN 乳腺癌 顺铂 化学治疗 

分 类 号：R737.9[医药卫生—肿瘤] R730.53[医药卫生—临床医学]