Rhodococcus sp.T3-1菌株降解乙草胺的特性  

Degradation characteristics of an acetochlor-degrading bacterium Rhodococcus sp.T3-1

作　　者：侯颖[1,2] 王飞[2,3] 董维亮[2] 崔中利[2] 

机构地区：[1]河南科技大学食品与生物工程学院,河南洛阳471003 [2]南京农业大学生命科学学院,农业部农业环境微生物重点开放实验室,江苏南京210095 [3]江西农业大学生物科学与工程学院,江西南昌330045

出　　处：《中国环境科学》2013年第10期1785-1790,共6页China Environmental Science

基　　金：江苏省杰出青年基金项目(BK2012029);科技部十二五科技支撑项目(2012BAD14B02-03);国家自然科学基金资助项目(31270095);农业部"948"项目(2011-Z21);河南科技大学人才基金(09001642);河南科技大学青年科学基金项目(2013N019)

摘　　要：以乙草胺为唯一碳源，通过摇瓶培养研究了Rhodococcus sp.T3—1对乙草胺的降解特性．结果表明，菌株T3—1降解乙草胺的最适温度为37℃，且其在pH值6～10的范围内对100mg／L乙草胺的降解率均在96％曲7％之间．该菌株在接种量为5％条件下，14h内可将200mg／L的乙草胺降解95.5％：乙草胺的降解速率与乙草胺初始浓度呈负相关，与菌株T3—1的初始接种量呈正相关．菌株T3-1还可以降解丁草胺，但不能降解丙草胺、异丙草胺和毗草胺．The degradation characteristics of acetochlor by Rhodococcus sp. T3-1 were studied by using acetochlor as sole carbon source and shaking-flask culture. Results showed that the optimum temperature for the degradation of acetochlor by strain T3-1was 37~C, and the degradation rate of 100mg/L acetochlor was in the range of 96%～97% within the range ofpH 6-10. Strain T3-1could degrade 95.5% of 200mg/L acetochlor in 14h with inoculum size 5%. The degradation rate of acetoehlor was negatively and positively correlated with the initial concentration of acetochlor and inoculum size of strain T3-1, respectively. Strain T3-1 could also degrade butachlor, but not pretilachlor, propisochlor and metazachlor.

关 键 词：乙草胺 红球菌属 降解特性 

分 类 号：X172[环境科学与工程—环境科学]