焦性没食子酸对铜绿微囊藻的氧化胁迫效应研究  

Study on oxidative damage to Microcystis aeruginosa mediated by allelochemical pyrogallic acid from submerged macrophytes

作　　者：孙雪梅[1,2] 刘碧云[1] 鲁志营[1,2] 吴振斌[1] 

机构地区：[1]中国科学院水生生物研究所,淡水生态与生物技术国家重点实验室,湖北武汉430072 [2]中国科学院大学,北京100049

出　　处：《中国环境科学》2013年第10期1835-1841,共7页China Environmental Science

基　　金：国家自然科学基金资助项目(51178452,50909091,31123001);国家“十二五”水专项(2012ZX07101007-005)

摘　　要：沉水植物穗花狐尾藻分泌的焦性没食子酸是一种抑藻效应较强的化感物质,为进一步研究其抑藻机理,分别于黑暗和光照条件下对铜绿微囊藻进行12h不同浓度焦性没食子酸的暴露,研究藻细胞内超氧阴离子、过氧化氢及羟自由基水平、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量以及细胞膜损伤的情况.结果显示,0.5mg/L焦性没食子酸能诱导藻细胞内超氧阴离子水平的显著性上升(P<0.01);过氧化氢及羟自由基水平、SOD活性均随焦性没食子酸浓度的升高而升高;MDA含量和用以表征细胞膜损伤情况的碘化丙啶(PI)荧光强度分别在0.5和1mg/L的焦性没食子酸暴露浓度下出现显著升高(P<0.05).综上,较低浓度的焦性没食子酸就能诱导藻细胞产生活性氧,进而引发脂质过氧化并造成藻细胞膜的损伤,这证实了氧化胁迫效应是化感物质抑藻的重要机理.Pyrogallic acid （PA） secreted by submerged macrophytes Myriophyllum spicatum is a potent allelochemical toward Microcystis aeruginosa. For studying the mechanism of the allelopathic effect, the intracellular O2←level, H2O2/ OH^＊ level, SOD activity, MDA level and percentage of membrane-injured cells were detected, after M..aeruginosa were exposed to PA for 12h under dark and light conditions respectively. The results showed that 0.5mg/L PA could induce generation of O2←significantly （P〈0.01）; H202/OH^＊ level and SOD activity both exhibited dose-dependent increases; and to MDA level and Prodium Iodide fluorescence（indication of membrane-injured cells）, significant increases were observed when exposed to 0.5and lmg/L PA correspondingly（P〈0.05）. The results indicated that a low concentration of PA could induce significant ROS generation and consequent lipid peroxidation and membrane injury in M.aeruginosa, which suggested that oxidative damage may be one of the important allelopathic mechanisms.

关 键 词：焦性没食子酸 铜绿微囊藻 超氧阴离子 过氧化氢 超氧化物歧化酶 丙二醛 细胞膜损伤 

分 类 号：X171.5[环境科学与工程—环境科学]