前列腺癌干细胞分离方法研究进展  

Advanced research on separating prostate cancer stem cells

作　　者：郝玉美[1] 贺欣[1] 宋娜玲[1] 

机构地区：[1]300192天津,北京协和医学院中国医学科学院放射医学研究所,天津市分子核医学重点实验室

出　　处：《国际放射医学核医学杂志》2013年第5期296-300,305,共6页International Journal of Radiation Medicine and Nuclear Medicine

基　　金：国家自然科学基金面上项目（81272511）;中国医学科学院放射医学研究所探索基金（ST1313）

摘　　要：前列腺癌是男性泌尿系统中常见的恶性肿瘤,极易发展为激素难治性前列腺癌而难以治愈.近年来的研究认为前列腺癌干细胞是前列腺癌发生、发展、转移和复发的根源,针对前列腺癌干细胞的靶向治疗可能是根治前列腺癌的有效途径,但这些细胞所占比例极小,几乎难以检测到,目前的研究难点就在于前列腺癌干细胞的分离技术效率较低.故拟深入研究前列腺癌干细胞的功能,首先必须有效地分离前列腺癌干细胞.该文就目前前列腺癌干细胞的几种主要分离方法的研究进展及存在的问题进行综述,包括基于前列腺癌干细胞表面标志物的荧光激活细胞分选法、磁性激活细胞分选法和基于前列腺癌干细胞生物特性的侧群细胞筛选法、体外无血清聚球筛选法.Prostate cancer is a common malignant tumor in male urinary system,and may easyly develope into the hormone refractory prostate cancer which can hardly be cured.Recent studies had found that the prostate cancer stem cells may be the source of the prostate cancer＇ s occurrence,development,metastasis and recurrence.The therapy targeting the prostate cancer stem cells may be the effective way to cure prostate cancer.But these cells is too low to be detected.The difficulty lies in the low separation efficiency of prostate cancer stem cell,so the effectively separating prostate cancer stem cells occupied the main position for the more in-depth research of prostate cancer stem cells.This paper reviews the research progress and existing problems on the several main separating methods of prostate cancer stem cells,includes the fluorescence activated cells sorting and magnetic activated cells sorting based on prostate cancer stem cell surface markers,the side-population sorting and serum-free medium sphere forming sorting based on prostate cancer stem cell＇s biology.

关 键 词：前列腺癌 前列腺癌干细胞 分离 

分 类 号：R737.25[医药卫生—肿瘤]