荸荠不同组培快繁方式的对比研究  

Comparison of Different Rapid Propagation Methods of Water Chestnut

作　　者：高美萍[1] 陈丽娟[1] 蔡炳华[1] 江文[1] 郭畅[1] 董伟清[1] 闭志强[1] 

机构地区：[1]广西农业科学院生物技术研究所,南宁530007

出　　处：《长江蔬菜》2013年第18期133-135,共3页Journal of Changjiang Vegetables

基　　金：国家科技支撑计划项目(2012BAD27B01-6);广西科技成果转化与推广计划(桂科转1346004-28);广西农科院重点项目(桂农科2012JZ04)

摘　　要：以广西农科院生物所选育的荸荠组培苗桂蹄2号为材料,利用间歇浸没式生物反应器(TIBs)与传统固体组培进行快繁培养,对比研究两种技术体系。研究结果表明,TIBs系统可使荸荠组织培养一代增殖40倍以上,远远高于传统方法,且培养出的植株分蘖多,叶状茎粗,生根少,长势好。Taking the tissue culture seedlings of Guiti No.2 as materials, we studied and compared two rapid propagation technical systems temporary immersion bioreactors system （TIBs） and traditional solid culture of water chestnut. The results showed that, the multiplication times of TIBs method was more than 40, which was much higher than that of traditional solid culture method, and the plants had more tillers, thicker cladophyll, less roots and grew well, providing a reference for the popularization and application of TIBs system in plant tissue culture.

关 键 词：荸荠 间歇浸没式生物反应器(TIBs) 固体组培 增殖 植株生长势 

分 类 号：S645.3[农业科学—蔬菜学]