水蒸气蒸馏与顶空进样GC-MS分析北柴胡挥发性成分  

Steam Distillation or Headspace Extraction Coupled to Gas Chromatography-Mass Spectrometry for Volatile Composition Analysis of Bupleurum chinense

作　　者：周严严[1] 巩丽丽[1] 孙国明[1] 仇霞[1] 曹原[1] 容蓉[1] 

机构地区：[1]山东中医药大学,山东济南250355

出　　处：《食品与药品》2013年第5期332-334,共3页Food and Drug

摘　　要：目的研究不同采集方法制备的北柴胡中挥发油化学成分。方法分别采用水蒸气蒸馏法和顶空进样技术提取北柴胡的挥发性成分,对其进行气相色谱-质谱(GC-MS)分析。结果两种提取方法无论在成分还是相对含量方面均有一定差异,采用水蒸气蒸馏法可鉴定36种挥发性化学成分,顶空进样技术可鉴定31种挥发性化学成分,共有成分为4种,且相对含量有差异。结论两种样品采集方法分别提供了不同沸点北柴胡挥发物的化学信息;运用这两种方法可建立更全面的北柴胡挥发性成分GC-MS表征体系。Objective To compare the difference of volatile components in Bupleurum chinense treated with different sample preparation methods. Methods Volatile components from Bupleurum chinense were extracted by steam distillation （SD） and headspace injection （HS） respectively and then analyzed by GC-MS. Results There was a certain difference between the volatile compounds extracted by the above two methods; 36 volatile chemical compounds were identified in volatile oil obtained by SD, whereas 31 volatile components were identified in the herb treated with HS; among the detected compounds in the samples treated with different methods, there were 4 mutual compounds with different relative contents. Conclusion The above two methods combined with GC-MS can be used for the analysis of volatile compounds in Bupleurum chinense and thus be feasible to establish GC-MS representation system for it.

关 键 词：北柴胡 挥发油 气相色谱-质谱联用 

分 类 号：R284.1[医药卫生—中药学]