新疆维吾尔族、汉族人群IL-10基因多态性与分泌量的相关性研究  

The study of IL-10 gene polymorphisms and contents relationship between Han and Uygur in Xinjiang

作　　者：朱雅婷[1] 魏娟[2] 张永军[2] 陈卫军[2] 李国昌[2] 

机构地区：[1]新疆医科大学附属肿瘤医院,乌鲁木齐830011 [2]石河子大学医学院第一附属医院,石河子832008

出　　处：《中国免疫学杂志》2013年第10期1022-1027,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(81060039)

摘　　要：目的:探讨IL-10基因启动子区-1082G/A(rs1800896)、-819C/T(rs1800871)、-592C/A(rs1800872)位点多态性在汉族和维吾尔族间的差异及与IL-10分泌量的关系。方法:采用聚合酶链反应及直接基因测序法比较124例汉族与131例维吾尔族健康人群基因型、等位基因频率和单倍体频率的差异。ELISA法测定两民族人群血清IL-10分泌水平。结果:IL-10-1082位点的AA、AG、GG基因型频率分别为:汉族(86.29%、12.9%、0.81%),维吾尔族(53.44%、41.98%、4.58%);-819位点的CC、CT、TT基因型频率分别为:汉族(7.26%、50.81%、41.93%),维吾尔族(24.43%、51.91%、23.66%);-592位点的CC、CA、AA基因型频率分别为:汉族(7.26%、50.81%、41.93%),维吾尔族(24.43%、51.91%、23.66%);汉族人群发现9种基因型和ACC、ATA、GCC、ACA、ATC五种单倍型,维吾尔族人群发现6种基因型和ACC、ATA、GCC三种单倍型;GCC型为高分泌型,ATA型为低分泌型,ACC型分泌量介于前两者之间。结论:血清IL-10的分泌水平在两民族间存在差异并受遗传因素影响;IL-10-1082G/A、-819C/T、-592C/A位点基因型频率和等位基因频率在汉族和维吾尔族人群间的分布均有显著性差异,新疆维吾尔族人群基因分布有其特点,这种不同可能是导致某些疾病在不同种族间发病率及预后差异的遗传因素之一。Objective： To investigate the IL-10 gene promoter-1082G/A （rs1800896）,-819C/T （rs1800871）, -592C/A （rs1800872） polymorphism in difference between Han and Uygur, while gene polymorphism relationship with IL-10 contents. Meth- ods： Using polymerase chain reaction and direct gene sequencing compare 124 cases of Han and 131 cases of Uighur healthy people the differences in genotypc, allele frequency and haplotype frequencies. Two ethnic populations of serum IL-10 contents were measured by ELISA. Results： The AA, AG, GG frequency of IL-10-1082 loci： Han （ 86.29% , 12.9% ,0.81% ） , Uygur（ 53.44% ,41.98% , 4. 58% ） respectively; the CC, CT, TT frequency of IL-10-819 loci： Han （7.26% ,50. 81% , 41.93% ） , Uygur （24.43% ,51.91% , 23.66% ） respectively; the CC, CA, AA fi＇equency of IL-10-592 loci： Hart （7.26% , 50. 81% , 41.93% ） , Uygur （24. 43% , 51.91% ,23.66% ） respectively; Han population have found nine genotypes and five haplotype which was ACC, ATA, GCC, ACA, ATU, and Uygur have found six genotypes and ACC, ATA, GCC haplotype; GCC was high-producer-type, ATA was low-producer- type, ACC was mid-producer-type. Conclusion： The level of IL-10 in serum was significant difference in Han and Uygur, it regulated by genetics. IL-10 gene in loci of-1082G/A,-819C/T, -592C/A had significant differences in genotypes and alleles frequency in Han and Uygur, the distribution of genotypes has its own characteristics in Xinjiang Uygur population, this difference may be the one of ge- netic factors which aftect the incidence and prognostic between different races.

关 键 词：白介素10 基因多态性 汉族 维吾尔族 

分 类 号：R394.5[医药卫生—医学遗传学]