温热对胃癌MKN-45细胞凋亡及基因Bax表达的影响  

The effect of hyperthermia chemotherapy on apoptosis and Bax gene expression of gastric cancer cells MKN-45

作　　者：蒋莉萍[1] 邱瑜[1] 肖凤[2] 吴凤珍[1] 

机构地区：[1]吉安市妇幼保健院病理科,江西吉安343000 [2]井冈山大学病理教研室,江西吉安343000

出　　处：《中国实验诊断学》2013年第10期1761-1764,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨温热对人胃癌细胞凋亡及基因Bax表达的影响。方法体外培养胃癌细胞MKN45并进行(43.0±0.1)℃温热疗法对癌细胞凋亡的影响;RT-PCR法检测温热后MKN-45细胞Bax mRNA水平的表达变化。结果温热后MKN-45细胞随着处理时间延长凋亡增加;RT-PCR结果显示,Bax mRNA的表达增加(P<0.05)。结论温热对胃癌MKN-45细胞具有明显的促凋亡作用,可能与其上调促凋亡基因Bax的表达有关。Objective To investigate the effect of hyperthermia chemotherapy on apoptosis and Bax gene expression of gastric cancer cells MKN-45. Methods Human gastric cancer cell lines MKN45 were cultured in vitro and under- went hyperthermic chemotherapy in （43.0±0.1）℃. The morphologic changes were observed by inverted microscope . The apoptosis rate was measured by TUNEL. Reverse transcription polymerase chain reaction （RT-PCR） were applied to detect Bax gene expression. Results After treatment with various times of hyperthermic chemotherapy of MKN-45 cells,The apopotosis gradually increased; RT-PCR results showed that Bax expression increased （P〈0.05）. Conclu- sion Hyperthermic chemotherapy to MKN45 cell has the obvious promoting apoptosis effect,may relevant with the in- creased expression of Bax gene.

关 键 词：MKN-45细胞 胃癌 温热 细胞凋亡 BAX mRNA 

分 类 号：R735.2[医药卫生—肿瘤]