副溶血弧菌的热激亚致死损伤与显微红外光谱检测  

Sublethal Thermal Treatment of Vibrio Parahaemolyticus and Its Determination by Micro-Fourier Transform Infrared Spectroscopy

作　　者：喻文娟[1,2] 施春雷[1] 刘玉敏 代敏[1] 史贤明[1] 

机构地区：[1]上海交通大学农业与生物学院食品科学与工程系中美食品安全联合研究中心 [2]分析测试中心,上海200240

出　　处：《分析化学》2013年第10期1470-1476,共7页Chinese Journal of Analytical Chemistry

基　　金：国家863项目(No.2012AA101601);国家科技支撑计划项目(No.2012BAD29B02);国家自然科学基金项目(No.31000063);上海市科委长三角科技联合攻关项目(No.11495810600)联合资助

摘　　要：采用传统的平板计数法作为对照，利用显微红外光谱技术（4000～400cm^-1）并结合化学计量学，研究了在55℃水浴中热激处理不同时间（0，2，4，6和8min）对副溶血弧菌失活和亚致死损伤的作用效果。二维主成分分析（PCA）表明，正常细菌与受损细菌能够各自聚类，明显区分，而且损伤程度不同的细菌也能够基本区分。载荷图分析（LPA）发现，加热处理后，副溶血弧菌中的多糖、结构蛋白、脂质、核酸都发生了变化，其细胞壁、细胞膜和DNA皆遭受损伤。类模拟软独立建模（SIMCA）的结果表明，一般情况下，受不同程度热损伤的细菌均有80％以上的预测率，能够被有效区别开。研究表明，显微红外光谱技术具有检测热激后亚致死损伤的副溶血弧菌的潜力。The inactivation and sublethal injury of Vibrio parahaemolyticus treatment was carried out at 55 ℃ （ for 0, 2, 4, 6 and 8 min, respectively） and determined by traditional microbiological tests and micro-Fourier transform infrared （FTIR） spectroscopy （4000-400 cm-1 ）. Two-dimensional principal component analysis （PCA） showed distinct segregation and clustering of heat-treated and untreated cells. The loadings for PC1, PC2 and PC3 confirmed that the content of polysaccharide, protein, lipid, and nucleic acid were significantly affected; cell wall, cell membrane and DNA were damaged during heating. Using soft independent modeling of class analogy （SIMCA）, the injury could be predicted with more than 80% accuracy with few exceptions. In conclusion, FT-IR spectroscopy has the potential to be applied in determining the presence of injured Vibrio parahaemolyticus cells that usually are underestimated or unrecoverable by conventional microbial techniques.

关 键 词：显微红外光谱技术 副溶血弧菌 亚致死 热激 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程] O657.33[轻工技术与工程—食品科学与工程]